PMID- 31226867
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 8
IP  - 2
DP  - 2019 Jun 20
TI  - Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine 
      Precision-Cut Lung Slices (PCLS) Models.
LID - 10.3390/pathogens8020082 [doi]
LID - 82
AB  - Respiratory infections caused by mycoplasma species in ruminants lead to 
      considerable economic losses. Two important ruminant pathogens are Mycoplasma 
      mycoides subsp. Mycoides (Mmm), the aetiological agent of contagious bovine 
      pleuropneumonia and Mycoplasma mycoides subsp. capri (Mmc), which causes 
      pneumonia, mastitis, arthritis, keratitis, and septicemia in goats. We 
      established precision cut lung slices (PCLS) infection model for Mmm and Mmc to 
      study host-pathogen interactions. We monitored infection over time using 
      immunohistological analysis and electron microscopy. Moreover, infection burden 
      was monitored by plating and quantitative real-time PCR. Results were compared 
      with lungs from experimentally infected goats and cattle. Lungs from healthy 
      goats and cattle were also included as controls. PCLS remained viable for up to 
      two weeks. Both subspecies adhered to ciliated cells. However, the titer of Mmm 
      in caprine PCLS decreased over time, indicating species specificity of Mmm. Mmc 
      showed higher tropism to sub-bronchiolar tissue in caprine PCLS, which increased 
      in a time-dependent manner. Moreover, Mmc was abundantly observed on pulmonary 
      endothelial cells, indicating partially, how it causes systemic disease. Tissue 
      destruction upon prolonged infection of slices was comparable to the in vivo 
      samples. Therefore, PCLS represents a novel ex vivo model to study host-pathogen 
      interaction in livestock mycoplasma.
FAU - Weldearegay, Yenehiwot Berhanu
AU  - Weldearegay YB
AUID- ORCID: 0000-0003-1700-4158
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      Yeneb.Welde@tiho-hannover.de.
FAU - Muller, Sandy
AU  - Muller S
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      Sandy.Mueller@tiho-hannover.de.
FAU - Hanske, Jana
AU  - Hanske J
AD  - Institute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute 
      for Animal Health, 17493 Greifswald-Insel Riems, Germany. 
      Jana.Haenske@lua.sms.sachsen.de.
FAU - Schulze, Anja
AU  - Schulze A
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      Anja.Schulze@tiho-hannover.de.
FAU - Kostka, Aline
AU  - Kostka A
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      aline.kostka@tiho-hannover.de.
FAU - Ruger, Nancy
AU  - Ruger N
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      nancy.rueger@tiho-hannover.de.
FAU - Hewicker-Trautwein, Marion
AU  - Hewicker-Trautwein M
AD  - Institute for Pathology, University of Veterinary Medicine Hannover, 30559 
      Hannover, Germany. Marion.Hewicker-Trautwein@tiho-hannover.de.
FAU - Brehm, Ralph
AU  - Brehm R
AUID- ORCID: 0000-0002-0489-7594
AD  - Institute of Anatomy, University of Veterinary Medicine Hannover, 30173 Hannover, 
      Germany. Ralph.Brehm@tiho-hannover.de.
FAU - Valentin-Weigand, Peter
AU  - Valentin-Weigand P
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      Peter.Valentin@tiho-hannover.de.
FAU - Kammerer, Robert
AU  - Kammerer R
AD  - Institute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute 
      for Animal Health, 17493 Greifswald-Insel Riems, Germany. Robert.Kammerer@fli.de.
FAU - Jores, Joerg
AU  - Jores J
AUID- ORCID: 0000-0003-3790-5746
AD  - Institute of Veterinary Bacteriology, University of Bern, CH-3001 Bern, 
      Switzerland. joerg.jores@vetsuisse.unibe.ch.
AD  - International Livestock Research Institute, PO Box 30709, 00100 Nairobi, Kenya. 
      joerg.jores@vetsuisse.unibe.ch.
FAU - Meens, Jochen
AU  - Meens J
AD  - Institute for Microbiology, Department of Infectious Diseases, University of 
      Veterinary Medicine Hannover, 30173 Hannover, Germany. 
      Jochen.Meens@tiho-hannover.de.
LA  - eng
GR  - Project no.: 13.1432.7.-001.00 Contract no.: 81170269/Bundesministerium fur 
      Wirtschaftliche Zusammenarbeit und Entwicklung/
GR  - Funding programme Open Access Publishing/Deutsche Forschungsgemeinschaft/
GR  - Funding programme Open Access Publishing/Stiftung Tierarztliche Hochschule 
      Hannover/
PT  - Journal Article
DEP - 20190620
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC6631151
OTO - NOTNLM
OT  - 3R
OT  - CBPP
OT  - MAKePS syndrome
OT  - Mycoplasma mycoides
OT  - PCLS
OT  - immunofluorescence
OT  - tropism
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2019/06/23 06:00
MHDA- 2019/06/23 06:01
PMCR- 2019/06/01
CRDT- 2019/06/23 06:00
PHST- 2019/05/29 00:00 [received]
PHST- 2019/06/13 00:00 [revised]
PHST- 2019/06/18 00:00 [accepted]
PHST- 2019/06/23 06:00 [entrez]
PHST- 2019/06/23 06:00 [pubmed]
PHST- 2019/06/23 06:01 [medline]
PHST- 2019/06/01 00:00 [pmc-release]
AID - pathogens8020082 [pii]
AID - pathogens-08-00082 [pii]
AID - 10.3390/pathogens8020082 [doi]
PST - epublish
SO  - Pathogens. 2019 Jun 20;8(2):82. doi: 10.3390/pathogens8020082.