PMID- 31228042
OWN - NLM
STAT- MEDLINE
DCOM- 20200212
LR  - 20220602
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 5
DP  - 2019 Oct
TI  - Caspase/AIF/apoptosis pathway: a new target of puerarin for diabetes mellitus 
      therapy.
PG  - 4787-4797
LID - 10.1007/s11033-019-04925-1 [doi]
AB  - Pancreatic beta cell damage is one of the crucial factors responsible for the 
      development of type 2 diabetes mellitus (T2DM). Previous studies have suggested 
      that puerarin (PR) could regulate the activities of the mitochondrial respiratory 
      chain complex in diabetic nephropathy (DN); however, whether PR can inhibit 
      pancreatic beta-cell apoptosis in T2DM remains to be elucidated. In the present 
      study, T2DM mice induced by high-fat diet and streptozotocin (STZ) injection were 
      used as a working model to investigate the mechanism of PR on pancreatic beta cell 
      apoptosis. The results showed that PR decreased the serum fasting blood glucose 
      (FBG), total cholesterol (TC), triglyceride (TG) and low-density lipoprotein 
      (LDL) levels but significantly increased the fasting blood insulin (FINS) and 
      high-density lipoprotein (HDL) levels. Furthermore, decreased caspase-3, 8, 9 and 
      apoptosis-inducing factor (AIF) proteins in the pancreas were detected by Western 
      blot analysis. Terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labelling (TUNEL) staining demonstrated that the pancreatic beta cell apoptosis was 
      inhibited by PR. Furthermore, PR improved the histopathological changes in 
      pancreatic tissue in T2DM mice. Collectively, the data show that PR can protect 
      the beta cells from apoptotic death in a mouse model of T2DM through regulating the 
      expression of apoptosis-related protein-AIF and caspase family proteins.
FAU - Liang, Tao
AU  - Liang T
AD  - College of Stomatology of Guangxi Medical University, No. 10, Shuangyong Road, 
      Nanning, 530021, People's Republic of China. kqliangtao@126.com.
FAU - Xu, Xiaohui
AU  - Xu X
AD  - Affiliated Tumor Hospital of Guangxi Medical University, Institute of Cancer 
      Prevention and Treatment of Guangxi Zhuang Autonomous Region, Nanning, 530021, 
      Guangxi, People's Republic of China.
FAU - Ye, Dongmei
AU  - Ye D
AD  - Department of Clinical Pharmacy, Hospital of Guangxi Zhuang Autonomous Region, 
      Nanning, 530021, Guangxi, People's Republic of China.
FAU - Chen, Wenxia
AU  - Chen W
AD  - College of Stomatology of Guangxi Medical University, No. 10, Shuangyong Road, 
      Nanning, 530021, People's Republic of China.
FAU - Gao, Biyun
AU  - Gao B
AD  - College of Stomatology of Guangxi Medical University, No. 10, Shuangyong Road, 
      Nanning, 530021, People's Republic of China.
FAU - Huang, Yanjun
AU  - Huang Y
AD  - Guangxi Medical University, Nanning, 530021, Guangxi, People's Republic of China.
LA  - eng
GR  - 2018GXNSFBA281037/Guangxi Natural Science Foundation/
GR  - 2018GXNSFBA281096/Guangxi Natural Science Foundation/
GR  - GXMUYSF201635/Youth Science Foundation of Guangxi Medical University/
GR  - 2018KY0128/Guangxi Middle School and Young Teachers' Basic Ability Improvement 
      Project/
PT  - Journal Article
DEP - 20190621
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Apoptosis Inducing Factor)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (Isoflavones)
RN  - 0 (Lipids)
RN  - EC 3.4.22.- (Caspases)
RN  - Z9W8997416 (puerarin)
SB  - IM
EIN - Mol Biol Rep. 2022 Apr;49(4):3369-3372. PMID: 35064411
MH  - Animals
MH  - Apoptosis/*drug effects/genetics
MH  - Apoptosis Inducing Factor/*metabolism
MH  - Biomarkers
MH  - Blood Glucose
MH  - Caspases/genetics/*metabolism
MH  - Diabetes Mellitus, Experimental
MH  - Diabetes Mellitus, Type 2/drug therapy/metabolism
MH  - Disease Models, Animal
MH  - Fasting
MH  - Gene Expression
MH  - Hypoglycemic Agents/chemistry/*pharmacology/therapeutic use
MH  - Insulin/blood
MH  - Isoflavones/chemistry/*pharmacology/therapeutic use
MH  - Lipids/blood
MH  - Mice
MH  - Pancreas/drug effects/metabolism/pathology/ultrastructure
MH  - Signal Transduction/*drug effects
PMC - PMC8782775
OTO - NOTNLM
OT  - Apoptosis-inducing factor
OT  - Caspase
OT  - Puerarin
OT  - Type 2 diabetes mellitus
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2019/06/23 06:00
MHDA- 2020/02/13 06:00
PMCR- 2019/06/21
CRDT- 2019/06/23 06:00
PHST- 2019/04/09 00:00 [received]
PHST- 2019/06/14 00:00 [accepted]
PHST- 2019/06/23 06:00 [pubmed]
PHST- 2020/02/13 06:00 [medline]
PHST- 2019/06/23 06:00 [entrez]
PHST- 2019/06/21 00:00 [pmc-release]
AID - 10.1007/s11033-019-04925-1 [pii]
AID - 4925 [pii]
AID - 10.1007/s11033-019-04925-1 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Oct;46(5):4787-4797. doi: 10.1007/s11033-019-04925-1. Epub 
      2019 Jun 21.