PMID- 31228822
OWN - NLM
STAT- MEDLINE
DCOM- 20190918
LR  - 20190918
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 181
DP  - 2019 Oct 15
TI  - In vitro assessment of effects of persistent organic pollutants on the 
      transactivation of estrogen receptor alpha and beta (ERalpha and ERbeta) from the Baikal seal 
      (Pusa sibirica).
PG  - 463-471
LID - S0147-6513(19)30671-2 [pii]
LID - 10.1016/j.ecoenv.2019.06.033 [doi]
AB  - To assess the effect of exposure to persistent organic pollutants (POPs) on the 
      estrogen receptor (ER) signaling pathway in Baikal seals (Pusa sibirica), we 
      investigated the molecular characterizations and functions of two Baikal seal ER 
      (bsER) isoforms, bsERalpha and bsERbeta. The bsERalpha and bsERbeta cDNA clones isolated have 
      an open reading frame of 595 and 530 amino acid residues, respectively. The 
      tissue distribution analyses of bsER mRNAs showed that bsERalpha transcripts were 
      primarily found in the ovary and uterus, and bsERbeta in the muscle in wild Baikal 
      seals. The immunofluorescence staining assay showed that 17beta-estradiol (E(2)) 
      treatment promoted the nuclear translocation of in vitro-expressed bsERalpha. 
      Transient transfection of bsERalpha in U2OS cells enhanced the transcription of pS2, 
      an ER target gene of E(2). We then measured bsER-mediated transactivation 
      potencies of POPs in an in vitro reporter gene assay system, in which a bsERalpha or 
      bsERbeta expression vector was transfected into COS-1 cells. For comparison, 
      transactivation potencies of POPs on mouse ERs (mERalpha and mERbeta) were also 
      evaluated in the same manner. Results showed significant dose-dependent responses 
      of bsERs and mERs when treated with p,p'-dichlorodiphenyltrichloroethane 
      (p,p'-DDT), and p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE). bsERs and mERs 
      showed no response when exposed to polychlorinated biphenyls (PCBs) or 
      2,3,7,8-tetrachlorodibenzo-p-dioxin. Comparison of the dose-response curves of 
      DDTs across species (bsERs vs. mERs) showed that bsERalpha had a response similar to 
      mERalpha, but bsERbeta was less sensitive than mERbeta. Comparing the lowest observable 
      effective concentrations of p,p'-DDT (2.8 muM) and p,p'-DDE (10 muM) for in vitro 
      bsERalpha-mediated transactivation with their hepatic concentrations in wild Baikal 
      seals indicated that some individuals accumulated these compounds at levels 
      comparable to the effective concentrations, suggesting the potential disruption 
      of the bsERalpha signaling pathway in the wild population by these compounds. 
      Co-transfection experiments with bsER and the aryl hydrocarbon receptor (AHR) 
      suggested that high accumulation of estrogenic compounds exerts a synergistic 
      effect with dioxin-like congeners on ER signaling through AHR activation in the 
      wild seal population.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Yoshinouchi, Yuka
AU  - Yoshinouchi Y
AD  - Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, 
      Matsuyama, 790-8577, Japan.
FAU - Shimizu, Sachiko
AU  - Shimizu S
AD  - Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, 
      Matsuyama, 790-8577, Japan.
FAU - Lee, Jin-Seon
AU  - Lee JS
AD  - Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, 
      Matsuyama, 790-8577, Japan.
FAU - Hirano, Masashi
AU  - Hirano M
AD  - Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, 
      Matsuyama, 790-8577, Japan.
FAU - Suzuki, Ken-Ichi T
AU  - Suzuki KT
AD  - Department of Mathematical and Life Sciences, Graduate School of Science, 
      Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8526, 
      Japan.
FAU - Kim, Eun-Young
AU  - Kim EY
AD  - Department of Life and Nanopharmaceutical Science and Department of Biology, 
      Kyung Hee University, Hoegi-Dong, Dongdaemun-Gu, Seoul, 130-701, Republic of 
      Korea.
FAU - Iwata, Hisato
AU  - Iwata H
AD  - Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, 
      Matsuyama, 790-8577, Japan. Electronic address: iwata.hisato.mz@ehime-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20190619
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Dioxins and Dioxin-like Compounds)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Dioxins and Dioxin-like Compounds/metabolism/*toxicity
MH  - Environmental Pollutants/metabolism/*toxicity
MH  - Estrogen Receptor alpha/*genetics
MH  - Estrogen Receptor beta/*genetics
MH  - Female
MH  - Mice
MH  - RNA, Messenger/metabolism
MH  - Seals, Earless
MH  - Transcriptional Activation/*drug effects
OTO - NOTNLM
OT  - Aryl hydrocarbon receptor
OT  - Baikal seal
OT  - Dichlorodiphenyltrichloroethane (DDT)
OT  - Estrogen receptor
OT  - Synergistic effect
EDAT- 2019/06/23 06:00
MHDA- 2019/09/19 06:00
CRDT- 2019/06/23 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/06/06 00:00 [revised]
PHST- 2019/06/11 00:00 [accepted]
PHST- 2019/06/23 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2019/06/23 06:00 [entrez]
AID - S0147-6513(19)30671-2 [pii]
AID - 10.1016/j.ecoenv.2019.06.033 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2019 Oct 15;181:463-471. doi: 
      10.1016/j.ecoenv.2019.06.033. Epub 2019 Jun 19.