PMID- 31228964
OWN - NLM
STAT- MEDLINE
DCOM- 20211025
LR  - 20220531
IS  - 1488-2353 (Electronic)
IS  - 0147-958X (Linking)
VI  - 42
IP  - 2
DP  - 2019 Jun 23
TI  - Golgi phosphoprotein-3 promotes invasiveness of gastric cancer cells through the 
      mTOR signalling pathway.
PG  - E38-47
LID - 10.25011/cim.v42i2.32815 [doi]
AB  - PURPOSE: Golgi phosphoprotein-3 (GOLPH3) is an oncogene that is overexpressed in 
      multiple cancers and is associated with poor prognosis. The aim of this study was 
      to examine the impact of GOLPH3 on the migration and metastasis of gastric cancer 
      cells. METHODS: Following the shRNA-mediated knockdown of GOLPH3, we analyzed 
      cytoskeletal reorganization and cell invasion, migration and adhesion, and 
      determined the impact of components of the mammalian target of the rapamycin 
      (mTOR) signalling pathway. RESULTS: The GOLPH3 mRNA and protein expression were 
      significantly lower in both SGC-7901 and MKN-28 cells as compared with 
      poorly-differentiated BGC-823 cells. The GOLPH3 knockdown also significantly 
      reduced cell invasion in all three cell lines through reduced migration as 
      compared with the non-targeting control sequence group. The GOLPH3 knockdown also 
      reduced F-actin in all three cell lines, and decreased cell adhesion in BGC-823 
      and SGC-7901 cells. Finally, p-mTOR, p70S6K, p-4EBP1 and RhoA protein levels were 
      significantly downregulated in shGOLPH3-1-treated cells. CONCLUSIONS: In 
      conclusion, GOLPH3 increased in poorly-differentiated gastric cancer cells, 
      activating the mTOR-70S6K/4EBP1-RhoA signalling pathway to promote the migration 
      and metastasis of gastric cancer cells.
FAU - Liu, Jun
AU  - Liu J
AD  - Department of Pathology, Tongde Hospital of Zhejiang Province, Hangzhou, China. 
      ymn168@126.com.
FAU - Wei, Hongquan
AU  - Wei H
FAU - Lai, Liqin
AU  - Lai L
FAU - Wang, Yuanyuan
AU  - Wang Y
FAU - Han, Xiaoyu
AU  - Han X
FAU - Zhang, Zhen
AU  - Zhang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190623
PL  - Canada
TA  - Clin Invest Med
JT  - Clinical and investigative medicine. Medecine clinique et experimentale
JID - 7804071
RN  - 0 (GOLPH3 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Membrane Proteins/genetics
MH  - Phosphoproteins/genetics
MH  - Signal Transduction
MH  - *Stomach Neoplasms/genetics
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
EDAT- 2019/06/24 06:00
MHDA- 2021/10/26 06:00
CRDT- 2019/06/24 06:00
PHST- 2019/06/23 00:00 [received]
PHST- 2019/06/24 06:00 [entrez]
PHST- 2019/06/24 06:00 [pubmed]
PHST- 2021/10/26 06:00 [medline]
AID - 10.25011/cim.v42i2.32815 [doi]
PST - epublish
SO  - Clin Invest Med. 2019 Jun 23;42(2):E38-47. doi: 10.25011/cim.v42i2.32815.