PMID- 31229921 OWN - NLM STAT- MEDLINE DCOM- 20200122 LR - 20211204 IS - 1950-6007 (Electronic) IS - 0753-3322 (Linking) VI - 117 DP - 2019 Sep TI - hsa_circ_0006168 sponges miR-100 and regulates mTOR to promote the proliferation, migration and invasion of esophageal squamous cell carcinoma. PG - 109151 LID - S0753-3322(19)31755-X [pii] LID - 10.1016/j.biopha.2019.109151 [doi] AB - Circle RNAs (circRNAs) are the novel noncoding RNAs with the covalent closed-loop structure, which play a crucial role in a variety of pathological processes, including cancer. Nevertheless, the expression profiles and functions of circRNAs in esophageal squamous cell cancer (ESCC) remain largely unknown. In this paper, 10 pairs of ESCC tissues were utilized to screen the circRNA expression profiles by means of microarray assay; further, a novel circular RNA named hsa_circ_0006168 was investigated. Meanwhile, the expression of hsa_circ_0006168 was measured in 52 ESCC tissues and in cell lines. Our results suggested that, hsa_circ_0006168 was remarkably increased not only in ESCC tissues but also in cell lines compared with those in normal cases. Besides, high hsa_circ_0006168 expression was positively connected with lymph node metastasis and TNM stage of ESCC patients. In vitro, the proliferation, invasion and migration capacities of ESCC cells were suppressed through down-regulating hsa_circ_0006168 expression. Besides, RNase R digestion assay confirmed that hsa_circ_0006168 was more stable than its linear CNOT6L mRNA form. Moreover, nuclear and cytoplasmic fraction assay indicated that hsa_circ_0006168 was mainly distributed in the cytoplasm of Kyse450 and TE13 cells. Mechanically, it was discovered in this study that hsa_circ_0006168 might regulate the expression of Mammalian Target of Rapamycin (mTOR) by sponging microRNA-100 (miR-100). Taken together, hsa_circ_0006168 can promote ESCC proliferation, migration and invasion through the competing endogenous RNA (ceRNA) mechanism, which has been first confirmed in our results. In ESCC, hsa_circ_0006168 can serve as a potential diagnostic biomarker and therapeutic target. CI - Copyright (c) 2019 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Shi, Yijun AU - Shi Y AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. FAU - Guo, Zizhang AU - Guo Z AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. FAU - Fang, Na AU - Fang N AD - Institute of Molecular Biology & Translational Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China. FAU - Jiang, Wei AU - Jiang W AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. FAU - Fan, Yu AU - Fan Y AD - Institute of Molecular Biology & Translational Medicine, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, Jiangsu, 212002, People's Republic of China. FAU - He, Yaozhou AU - He Y AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. FAU - Ma, Zijian AU - Ma Z AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. FAU - Chen, Yijiang AU - Chen Y AD - Department of Thoracic and Cardiovascular Surgery, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, People's Republic of China. Electronic address: chenyjnjmu@163.com. LA - eng PT - Journal Article DEP - 20190621 PL - France TA - Biomed Pharmacother JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JID - 8213295 RN - 0 (MIRN100 microRNA, human) RN - 0 (MicroRNAs) RN - EC 2.7.1.1 (MTOR protein, human) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) SB - IM MH - Base Sequence MH - Cell Line, Tumor MH - Cell Movement/*genetics MH - Cell Proliferation/genetics MH - Down-Regulation/genetics MH - Esophageal Squamous Cell Carcinoma/*genetics/*pathology MH - Female MH - Gene Expression Regulation, Neoplastic MH - Humans MH - Male MH - MicroRNAs/genetics/*metabolism MH - Middle Aged MH - Neoplasm Invasiveness MH - Neoplasm Metastasis MH - TOR Serine-Threonine Kinases/*metabolism MH - Up-Regulation/genetics OTO - NOTNLM OT - Circular RNA OT - ESCC OT - Esophageal squamous cell carcinoma OT - hsa_circ_0006168 OT - mTOR OT - miR-100 EDAT- 2019/06/24 06:00 MHDA- 2020/01/23 06:00 CRDT- 2019/06/24 06:00 PHST- 2019/04/18 00:00 [received] PHST- 2019/06/14 00:00 [revised] PHST- 2019/06/17 00:00 [accepted] PHST- 2019/06/24 06:00 [pubmed] PHST- 2020/01/23 06:00 [medline] PHST- 2019/06/24 06:00 [entrez] AID - S0753-3322(19)31755-X [pii] AID - 10.1016/j.biopha.2019.109151 [doi] PST - ppublish SO - Biomed Pharmacother. 2019 Sep;117:109151. doi: 10.1016/j.biopha.2019.109151. Epub 2019 Jun 21.