PMID- 312304
OWN - NLM
STAT- MEDLINE
DCOM- 19790728
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 26
IP  - 1
DP  - 1979
TI  - Identification and quantitation of B and T cells by cytofluorographic analysis.
PG  - 47-60
AB  - With cytofluorographic analysis (CFGA) of cells stained with the fluorescent dye 
      acridine orange (AO), the major peripheral white cell populations--lymphocytes, 
      monocytes, and polymorphonuclear cells--display different characteristics and 
      appear as distinct populations which can be quantitated. In this study we present 
      a method for determining percentages of human T and B cells lymphocyte 
      subpopulations by CFGA and display of the data on a computer-generated 
      3-dimensional grid. Lymphocytes were depleted of either B, T, or both B and T 
      cells by rosetting with erythrocytes and separated by centrifugation. The B cell 
      and T cell depleted and non-rosetting cell subpopulations localized on constant, 
      distinct areas of the display grid. The percentages of T and B cells in 
      peripheral blood samples from 6 normals analyzed by CFGA did not differ from the 
      results obtained by light microscope counting (LMC).
FAU - Beveridge, R A
AU  - Beveridge RA
FAU - Super, B S
AU  - Super BS
FAU - Chretien, P B
AU  - Chretien PB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
SB  - IM
MH  - Adult
MH  - B-Lymphocytes/*immunology
MH  - Cell Separation
MH  - Humans
MH  - Middle Aged
MH  - Photofluorography
MH  - Rosette Formation
MH  - T-Lymphocytes/*immunology
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
AID - 0022-1759(79)90040-1 [pii]
AID - 10.1016/0022-1759(79)90040-1 [doi]
PST - ppublish
SO  - J Immunol Methods. 1979;26(1):47-60. doi: 10.1016/0022-1759(79)90040-1.