PMID- 31231909
OWN - NLM
STAT- MEDLINE
DCOM- 20200819
LR  - 20200819
IS  - 1365-2303 (Electronic)
IS  - 0956-5507 (Linking)
VI  - 30
IP  - 6
DP  - 2019 Nov
TI  - Cytopathological spectrum of peripheral neuroblastic tumours in fine needle 
      aspiration cytology and categorisation as per International Neuroblastoma 
      Pathology Classification.
PG  - 634-643
LID - 10.1111/cyt.12747 [doi]
AB  - OBJECTIVE: The aim of this analysis was to describe the cytopathology spectrum of 
      peripheral neuroblastic tumours (NTs) including neuroblastoma (NB), 
      ganglioneuroblastoma (GNB) and ganglioneuroma (GN). Feasibility of applying the 
      International Neuroblastoma Pathology Classification (INPC) to further subtype 
      NTs in cytology was evaluated. METHODS: All peripheral NTs reported on fine 
      needle aspiration during 2011-2015 were retrieved and detailed cytomorphological 
      evaluation was performed. Based on INPC criteria, NBs were further categorised as 
      undifferentiated, poorly differentiated and differentiating subtypes. 
      Mitotic-karyorrhectic index was evaluated. Immunocytochemistry on cell blocks was 
      reviewed wherever available. MYCN amplification by fluorescence in situ 
      hybridisation was performed in 11 cases on smears. RESULTS: A total of 90 cases 
      including 83 NBs, six GNB and one GN were evaluated. The age range was 
      12 days-12 years, with 55 males and 45 females. Both the primary and metastatic 
      locations were aspirated. Applying the INPC criteria, there were 61 poorly 
      differentiated, 14 undifferentiated, eight differentiating NB and six GNB. 
      Immunocytochemistry on cell blocks showed positivity for at least two neuronal 
      markers in NB. Mitotic-karyorrhectic index was high in 63, low in 22 and 
      intermediate in two cases, respectively. MYCN amplification by fluorescence in 
      situ hybridisation was feasible on smears and was amplified in 6 out of 11 cases 
      tested. CONCLUSION: Peripheral NT types including NB, GNB and GN have distinctive 
      cytomorphology. NBs can be further subtyped as undifferentiated, poorly 
      differentiated and differentiating subtypes as per INPC criteria.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Koshy, Abin
AU  - Koshy A
AD  - Department of Cytology and Gynecological Pathology, Postgraduate Institute of 
      Medical Education and Research, Chandigarh, India.
FAU - Jain, Richa
AU  - Jain R
AD  - Hemato-Oncology Unit, Department of Pediatrics, Postgraduate Institute of Medical 
      Education and Research, Chandigarh, India.
FAU - Srinivasan, Radhika
AU  - Srinivasan R
AUID- ORCID: 0000-0002-1771-091X
AD  - Department of Cytology and Gynecological Pathology, Postgraduate Institute of 
      Medical Education and Research, Chandigarh, India.
FAU - Bhatia, Prateek
AU  - Bhatia P
AD  - Hemato-Oncology Unit, Department of Pediatrics, Postgraduate Institute of Medical 
      Education and Research, Chandigarh, India.
FAU - Kakkar, Nandita
AU  - Kakkar N
AD  - Department of Histopathology, Postgraduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Rajwanshi, Arvind
AU  - Rajwanshi A
AD  - Department of Cytology and Gynecological Pathology, Postgraduate Institute of 
      Medical Education and Research, Chandigarh, India.
FAU - Gupta, Nalini
AU  - Gupta N
AUID- ORCID: 0000-0003-3385-1955
AD  - Department of Cytology and Gynecological Pathology, Postgraduate Institute of 
      Medical Education and Research, Chandigarh, India.
FAU - Dey, Pranab
AU  - Dey P
AUID- ORCID: 0000-0001-9297-5400
AD  - Department of Cytology and Gynecological Pathology, Postgraduate Institute of 
      Medical Education and Research, Chandigarh, India.
FAU - Trehan, Amita
AU  - Trehan A
AD  - Hemato-Oncology Unit, Department of Pediatrics, Postgraduate Institute of Medical 
      Education and Research, Chandigarh, India.
FAU - Bansal, Deepak
AU  - Bansal D
AD  - Hemato-Oncology Unit, Department of Pediatrics, Postgraduate Institute of Medical 
      Education and Research, Chandigarh, India.
LA  - eng
PT  - Journal Article
DEP - 20190725
PL  - England
TA  - Cytopathology
JT  - Cytopathology : official journal of the British Society for Clinical Cytology
JID - 9010345
SB  - IM
MH  - Biopsy, Fine-Needle/*methods
MH  - Child
MH  - Child, Preschool
MH  - Cytodiagnosis/methods
MH  - Diagnosis, Differential
MH  - Female
MH  - Ganglioneuroblastoma/classification/*diagnosis/pathology
MH  - Ganglioneuroma/classification/*diagnosis/pathology
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Neuroblastoma/classification/*diagnosis/pathology
MH  - Prognosis
OTO - NOTNLM
OT  - International Neuroblastoma Pathology Classification
OT  - MYCN amplification
OT  - fine needle aspiration
OT  - ganglioneuroblastoma
OT  - neuroblastoma
OT  - peripheral neuroblastic tumours
EDAT- 2019/06/25 06:00
MHDA- 2020/08/20 06:00
CRDT- 2019/06/25 06:00
PHST- 2019/01/17 00:00 [received]
PHST- 2019/05/01 00:00 [revised]
PHST- 2019/06/08 00:00 [accepted]
PHST- 2019/06/25 06:00 [pubmed]
PHST- 2020/08/20 06:00 [medline]
PHST- 2019/06/25 06:00 [entrez]
AID - 10.1111/cyt.12747 [doi]
PST - ppublish
SO  - Cytopathology. 2019 Nov;30(6):634-643. doi: 10.1111/cyt.12747. Epub 2019 Jul 25.