PMID- 31234172
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20190906
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 158
IP  - 3
DP  - 2019
TI  - Chromosomal Localization of 18S-28S rDNA and (TTAGGG)n Sequences in Two South 
      African Dormice of the Genus Graphiurus (Rodentia: Gliridae).
PG  - 145-151
LID - 10.1159/000500985 [doi]
AB  - Classical cytogenetics and mapping of 18S-28S rDNA and (TTAGGG)n sequences by 
      fluorescence in situ hybridization (FISH) was performed on Graphiurus platyops 
      (GPL) and Graphiurus ocularis (GOC) metaphases with the aim to characterize the 
      genomes. In both species, inverted DAPI karyotypes showed the same diploid 
      number, 2n = 46, and hybridization of the (TTAGGG)n probe revealed interstitial 
      telomeric sequences (ITSs) at the centromeres of almost all bi-armed chromosomes. 
      FISH with the rDNA probe localized nucleolus organizer regions (NORs), at the 
      terminal ends of the p arms of the subtelocentric pairs 16 and 17 in both species 
      and detected additional signals on GPL8 and GOC18, 19, and 22. The species have 
      similar karyotypes, but their chromosome pairs 18-22 differ in morphology; these 
      are acrocentric in G. platyops, as also confirmed by C-banding, and 
      subtelocentric in G. ocularis. These differences in pairs 18-22 were also 
      highlighted by hybridization of the telomeric probe (TTAGGG)n, which showed the 
      small p arms in G. ocularis enriched with ITSs. FISH of rDNA probes detected 
      multiple NOR loci in G. ocularis, underlining the intense evolutionary dynamics 
      related to these genes. Although the Graphiurus species analyzed have similar 
      karyotypes, the results on the repetitive sequences indicate a complex pattern of 
      genomic reorganization and evolution occurring in these phylogenetically close 
      species.
CI  - (c) 2019 S. Karger AG, Basel.
FAU - Milioto, Vanessa
AU  - Milioto V
FAU - Vlah, Sara
AU  - Vlah S
FAU - Mazzoleni, Sofia
AU  - Mazzoleni S
FAU - Rovatsos, Michail
AU  - Rovatsos M
FAU - Dumas, Francesca
AU  - Dumas F
LA  - eng
PT  - Journal Article
DEP - 20190622
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 28S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Banding
MH  - Chromosomes, Mammalian/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Myoxidae/*genetics
MH  - RNA, Ribosomal, 18S/*genetics
MH  - RNA, Ribosomal, 28S/*genetics
MH  - South Africa
OTO - NOTNLM
OT  - Chromosomal evolution
OT  - Fusion
OT  - Graphiurus ocularis
OT  - Graphiurus platyops
OT  - Karyotypes
OT  - Repetitive sequence amplification
EDAT- 2019/06/25 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/25 06:00
PHST- 2019/03/06 00:00 [accepted]
PHST- 2019/06/25 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2019/06/25 06:00 [entrez]
AID - 000500985 [pii]
AID - 10.1159/000500985 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2019;158(3):145-151. doi: 10.1159/000500985. Epub 2019 Jun 
      22.