PMID- 31234788
OWN - NLM
STAT- MEDLINE
DCOM- 20200403
LR  - 20200403
IS  - 1471-2180 (Electronic)
IS  - 1471-2180 (Linking)
VI  - 19
IP  - 1
DP  - 2019 Jun 24
TI  - A comparative study of microbial community and dynamics of Asaia in the brown 
      planthopper from susceptible and resistant rice varieties.
PG  - 139
LID - 10.1186/s12866-019-1512-9 [doi]
LID - 139
AB  - BACKGROUND: The brown planthopper (BPH) is likely the most destructive, piercing 
      and sucking monophagous insect pest of rice that causes substantial economic 
      losses to farmers. Although yeast-like symbionts (YLS) and virus transmission 
      have been observed in the BPH, the bacterial population inhabiting the BPH has 
      received minimal research attention. Labelling BPH-associated bacterial species 
      may shed light on BPH biology and the interaction between the BPH and rice to 
      provide novel approaches for the efficient control of this insect pest. RESULTS: 
      We examined RNA-seq results to identify bacterial populations present in 
      different generations of BPHs maintained on susceptible or resistant rice 
      varieties. Overall, 87 operational taxonomic units (OTUs) were determined from 
      the BPH-F0, F6 and F16 generations. These OTUs had Shannon and Simpson index 
      values of 0.37-0.6 and 0.56-1.19, respectively. The evenness values of 0.7-1.00 
      showed the vastness of the bacterial diversity recovered from the BPH samples. 
      The results showed high species diversity in the BPHs collected from susceptible 
      rice and a high number of members of unclassified bacteria in the BPHs isolated 
      from resistant rice. We noticed that Proteobacteria OTUs were predominant across 
      all samples. Furthermore, PCR data of Asaia species showed variable DNA 
      amplification across the BPH samples collected from susceptible or resistant 
      varieties. The identification of Asaia in BPH eggs and BPH-egg-infected rice 
      revealed its influence on the interaction between the BPH egg and rice. 
      CONCLUSIONS: The BPHs had clear differences in their microbiomes and in their 
      ability to feed on different rice hosts. These variations could have an essential 
      impact on host adaptation and interaction. These results provide a better 
      understanding of the bacterial diversity and interaction of the microbiome of 
      different generations of BPHs. Furthermore, PCR data of Asaia sp. variation 
      across the BPH samples (isolated from different host genotypes selected from the 
      field and laboratory, including BPH eggs and egg-infected rice tissues), suggest 
      that Asaia could be an important member of the insect microbiome involved in 
      adaptation, its interaction with rice and, most importantly, as a paratransgenic 
      tool for insect control.
FAU - Ojha, Abhishek
AU  - Ojha A
AD  - State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510275, Guangdong, China.
AD  - State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural 
      University, Hefei, 230036, Anhui, China.
FAU - Zhang, Wenqing
AU  - Zhang W
AUID- ORCID: 0000-0002-1577-1225
AD  - State Key Laboratory of Biocontrol and School of Life Sciences, Sun Yat-sen 
      University, Guangzhou, 510275, Guangdong, China. lsszwq@mail.sysu.edu.cn.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190624
PL  - England
TA  - BMC Microbiol
JT  - BMC microbiology
JID - 100966981
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animal Feed
MH  - Animals
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Disease Resistance
MH  - Hemiptera/*microbiology/pathogenicity
MH  - Microbiota
MH  - Oryza/*growth & development/parasitology
MH  - Phylogeny
MH  - Plant Diseases/parasitology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, RNA/*methods
PMC - PMC6591912
OTO - NOTNLM
OT  - 16S rRNA
OT  - Diversity
OT  - Insect-plant interaction
OT  - Macropterous and brachypterous BPH
OT  - Microbiome
OT  - Operational taxonomic units
COIS- The authors declare that they have no competing interests.
EDAT- 2019/06/27 06:00
MHDA- 2020/04/04 06:00
PMCR- 2019/06/24
CRDT- 2019/06/26 06:00
PHST- 2019/01/17 00:00 [received]
PHST- 2019/06/10 00:00 [accepted]
PHST- 2019/06/26 06:00 [entrez]
PHST- 2019/06/27 06:00 [pubmed]
PHST- 2020/04/04 06:00 [medline]
PHST- 2019/06/24 00:00 [pmc-release]
AID - 10.1186/s12866-019-1512-9 [pii]
AID - 1512 [pii]
AID - 10.1186/s12866-019-1512-9 [doi]
PST - epublish
SO  - BMC Microbiol. 2019 Jun 24;19(1):139. doi: 10.1186/s12866-019-1512-9.