PMID- 31237476
OWN - NLM
STAT- MEDLINE
DCOM- 20191031
LR  - 20211204
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 32
IP  - 11
DP  - 2019 Nov
TI  - TaXa21, a Leucine-Rich Repeat Receptor-Like Kinase Gene Associated with TaWRKY76 
      and TaWRKY62, Plays Positive Roles in Wheat High-Temperature Seedling Plant 
      Resistance to Puccinia striiformis f. sp. tritici.
PG  - 1526-1535
LID - 10.1094/MPMI-05-19-0137-R [doi]
AB  - Puccinia striiformis f. sp. tritici causes wheat stripe rust, one of most 
      important diseases of wheat worldwide. High-temperature seedling plant (HTSP) 
      resistance of wheat to P. striiformis f. sp. tritici is one specific type of host 
      resistance, induced by high temperature (HT). Receptor-like kinases (RLKs) play 
      key roles in regulating plant development and signaling networks, but there have 
      been no reports on possible roles played by RLKs in wheat HTSP to P. striiformis 
      f. sp. tritici. In the present study, a leucine rich repeat (LRR)-RLK gene, 
      TaXa21, with a high homology with rice bacterial blight resistance gene Xa21, was 
      cloned from wheat cultivar Xiaoyan 6 (XY 6). TaXa21 expression was up-regulated 
      by the exposure to HT (20 degrees C) for 24 h at 8 days postinoculation with P. 
      striiformis f. sp. tritici and was induced by ethylene (ET) and hydrogen peroxide 
      (H(2)O(2)). Knocking down TaXa21 using virus-induced gene silencing reduced HTSP 
      resistance to P. striiformis f. sp. tritici compared with the control plants. In 
      addition, the expression level of TaCAT in the H(2)O(2) pathway was induced and 
      TaACO in the ET signal pathway was reduced in the HT-treated TaXa21-silenced 
      plants. Transient expression of TaXa21 in tobacco leaves confirmed its 
      subcellular localization in plasma membrane, consistent with the prediction from 
      bioinformatics analysis. The transmembrane and kinase domain of TaXa21 can 
      interact with TaWRKY76 in the nucleus and cell membrane, which is different from 
      the localization of Xa21 in rice. The interaction between TaWRKY76 and TaWRKY62 
      (positively involved in the HTSP resistance of XY 6) were observed. Together, 
      these results indicated that TaXa21 is a RLK associated with TaWRKY76 and 
      TaWRKY62 and functions as a positive regulator of wheat HTSP resistance to P. 
      striiformis f. sp. tritici. Furthermore, the host defense is mediated by the 
      H(2)O(2) and ET signal pathways.
FAU - Wang, Jiahui
AU  - Wang J
AUID- ORCID: 0000-0003-1642-9103
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi 712100, 
      China.
FAU - Wang, Junjuan
AU  - Wang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi 712100, 
      China.
FAU - Shang, Hongsheng
AU  - Shang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi 712100, 
      China.
FAU - Chen, Xianming
AU  - Chen X
AUID- ORCID: 0000-0002-9013-5974
AD  - Agricultural Research Service, United States Department of Agriculture and 
      Department of Plant Pathology, Washington State University, Pullman, WA 
      99164-6430, U.S.A.
FAU - Xu, Xiangming
AU  - Xu X
AD  - NIAB East Malling Research, New Road, East Malling, ME19 6BJ, Kent, U.K.
FAU - Hu, Xiaoping
AU  - Hu X
AUID- ORCID: 0000-0002-8155-7040
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Taicheng Road 3, Yangling, Shaanxi 712100, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20190917
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - *Basidiomycota/physiology
MH  - *Disease Resistance/genetics
MH  - Hot Temperature
MH  - *Protein Serine-Threonine Kinases/metabolism
MH  - Seedlings/microbiology
MH  - *Triticum/enzymology/genetics/microbiology
OTO - NOTNLM
OT  - TaWRKY62
OT  - TaWRKY76
OT  - TaXa21
OT  - high-temperature seedling plant resistance
OT  - interaction
OT  - stripe rust
EDAT- 2019/06/27 06:00
MHDA- 2019/11/02 06:00
CRDT- 2019/06/26 06:00
PHST- 2019/06/27 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2019/06/26 06:00 [entrez]
AID - 10.1094/MPMI-05-19-0137-R [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2019 Nov;32(11):1526-1535. doi: 
      10.1094/MPMI-05-19-0137-R. Epub 2019 Sep 17.