PMID- 31238210
OWN - NLM
STAT- MEDLINE
DCOM- 20200131
LR  - 20211204
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 181
DP  - 2019 Sep 1
TI  - Nanoengineered chlorin e6 conjugated with hydrogel for photodynamic therapy on 
      cancer.
PG  - 778-788
LID - S0927-7765(19)30446-1 [pii]
LID - 10.1016/j.colsurfb.2019.06.040 [doi]
AB  - The aim of the present study is to fabricate hydrogel as a photosensitizer (PS) 
      for photodynamic therapy. Chlorin e6 (Ce6)-fucoidan/alginates@gellam gum 
      (Ce6-Fu/AL@GG)-based hydrogel was fabricated and characterised in terms of 
      morphology and functional groups. MTT assay was used to check toxicity and also 
      performed scratch assay for wound healing property of Ce6-Fu/AL@GGH. Fourier 
      transform infrared spectroscopy (FT-IR) confirmed the existence of physical 
      interactions between polysaccharides. Thermogravimetric analysis (TGA) and 
      Differential scanning calorimetry (DSC) analysis confirmed a decrease in the 
      thermal stability of the fabricated hydrogel. Scanning electron microscope (SEM) 
      and Transmission electron microscope (TEM) images demonstrated porous matrixes 
      representing homogeneous dispersion of nanoparticles in the hydrogel. 
      Cytotoxicity tests revealed that a decrease in the cell viability occurred in PDT 
      after 48 h treatment. Both Ce6 and laser irradiation induced the HT-29 apoptotic 
      effect that was mediated by intracellular ROS generation and mitochondrial 
      damage. The laser-treated hydrogel was effective in inhibiting HT-29 cell growth. 
      Ce6-Fu/AL@GG hydrogel can be a promising platform for PDT on cancer treatment.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Karuppusamy, Shanmugapriya
AU  - Karuppusamy S
AD  - Department of Biomedical Engineering and Center for Marine-Integrated Biomedical 
      Technology (BK 21 Plus), Pukyong National University, Busan, South Korea.
FAU - Hyejin, Kim
AU  - Hyejin K
AD  - Interdisciplinary program of Biomedical Mechanical & Electrical Engineering, 
      Pukyong National University, Busan, South Korea.
FAU - Kang, Hyun Wook
AU  - Kang HW
AD  - Department of Biomedical Engineering and Center for Marine-Integrated Biomedical 
      Technology (BK 21 Plus), Pukyong National University, Busan, South Korea; 
      Interdisciplinary program of Biomedical Mechanical & Electrical Engineering, 
      Pukyong National University, Busan, South Korea. Electronic address: 
      wkang@pukyong.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20190619
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Chlorophyllides)
RN  - 0 (Hydrogels)
RN  - 0 (Photosensitizing Agents)
RN  - 0 (Porphyrins)
RN  - 0 (Reactive Oxygen Species)
RN  - 5S2CCF3T1Z (phytochlorin)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Chlorophyllides
MH  - Colonic Neoplasms/*drug therapy/pathology
MH  - Drug Screening Assays, Antitumor
MH  - HT29 Cells
MH  - Humans
MH  - Hydrogels/chemistry/*pharmacology
MH  - Particle Size
MH  - *Photochemotherapy
MH  - Photosensitizing Agents/chemistry/*pharmacology
MH  - Porphyrins/chemistry/*pharmacology
MH  - Reactive Oxygen Species/analysis/metabolism
MH  - Surface Properties
MH  - Tumor Cells, Cultured
MH  - Wound Healing/drug effects
OTO - NOTNLM
OT  - Anticancer
OT  - Chlorin e6
OT  - Hydrogel
OT  - Photodynamic therapy
OT  - Polysaccharides
OT  - ROS generation
EDAT- 2019/06/27 06:00
MHDA- 2020/02/01 06:00
CRDT- 2019/06/26 06:00
PHST- 2019/04/05 00:00 [received]
PHST- 2019/05/21 00:00 [revised]
PHST- 2019/06/18 00:00 [accepted]
PHST- 2019/06/27 06:00 [pubmed]
PHST- 2020/02/01 06:00 [medline]
PHST- 2019/06/26 06:00 [entrez]
AID - S0927-7765(19)30446-1 [pii]
AID - 10.1016/j.colsurfb.2019.06.040 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2019 Sep 1;181:778-788. doi: 
      10.1016/j.colsurfb.2019.06.040. Epub 2019 Jun 19.