PMID- 31238801 OWN - NLM STAT- MEDLINE DCOM- 20191021 LR - 20200625 IS - 1943-4936 (Electronic) IS - 1040-6387 (Print) IS - 1040-6387 (Linking) VI - 31 IP - 4 DP - 2019 Jul TI - Effects of marked hypertriglyceridemia and lipid clearance techniques on canine biochemistry testing. PG - 546-553 LID - 10.1177/1040638719858690 [doi] AB - Triglyceride concentrations in dogs with hyperlipidemic disorders can exceed concentrations used by assay manufacturers for interference testing. High-speed centrifugation or the polar solvent LipoClear reduce triglyceride concentrations, but efficacy requires evaluation in veterinary species. We determined the effect of marked hypertriglyceridemia on canine biochemistry testing; assessed the ability of high-speed centrifugation or LipoClear to correct lipemic interferences; and determined if LipoClear introduces inaccuracy into biochemistry assays. Fifteen pooled canine serum samples were aliquoted and spiked with equal volumes of water or Intralipid [triglyceride concentration 33.9 mmol/L (3,000 mg/dL)]. Intralipid aliquots underwent lipid removal by high-speed centrifugation or LipoClear treatment, and a water-spiked aliquot underwent LipoClear treatment. Biochemistry panels were performed using a Vitros 4600 chemistry analyzer. Results were compared by paired t-test or Wilcoxon test. Total observed errors were considered clinically acceptable if below veterinary allowable total error (TEa) guidelines. Statistically significant (p