PMID- 31241217
OWN - NLM
STAT- MEDLINE
DCOM- 20200908
LR  - 20200908
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 11
DP  - 2019 Nov
TI  - Circular RNA circPIP5K1A promotes non-small cell lung cancer proliferation and 
      metastasis through miR-600/HIF-1alpha regulation.
PG  - 19019-19030
LID - 10.1002/jcb.29225 [doi]
AB  - Circular RNAs (circRNAs) have an important function in human diseases, especially 
      in cancer. circRNA hsa_circ_0014130 (circPIP5K1A), a particularly abundant 
      circRNA, participates in the tumorigenesis of non-small cell lung cancer (NSCLC), 
      although the underlying regulatory mechanism remains unclear. Here, we 
      investigated the circPIP5K1A role in NSCLC. Expression of circPIP5K1A in NSCLC 
      cell lines was explored with quantitative real-time PCR. The effect of 
      circPIP5K1A on NSCLC was evaluated with circPIP5K1A silencing, miR-600 mimic 
      transfection, and hypoxia-inducible factor (HIF)-1alpha overexpression, followed by 
      assessment of cell proliferation, metastasis, and tumorigenesis in nude mice. The 
      subcellular localization of circPIP5K1A was evaluated via fluorescence in situ 
      hybridization (FISH), and correlation between circPIP5K1A, miR-600, and HIF-1alpha 
      was assessed by luciferase assay. The data demonstrated that circPIP5K1A 
      expression was increased in NSCLC cells. FISH showed that circPIP5K1A localized 
      to the cytoplasm. The circPIP5K1A knockdown suppressed NSCLC cell metastasis and 
      proliferation by promoting expression of miR-600. Overexpression of miR-600 
      inhibited HIF-1alpha-mediated metastasis and proliferation of NSCLC cell by 
      downregulating the endothelial mesenchymal transition-related proteins, Snail and 
      vimentin, and upregulating E-cadherin. In vivo experiments illustrated that 
      circPIP5K1A silence suppressed tumor growth and pulmonary metastasis. The 
      circPIP5K1A may function as an miR-600 sponge to facilitate NSCLC proliferation 
      and metastasis by promoting HIF-1alpha. A bifluorescein reporter experiment confirmed 
      that miR-600 was the circPIP5K1A target, and miR-600 interacted with the 3' 
      untranslated region of HIF-1alpha. These results show that circPIP5K1A acted as a 
      tumor promoter through a novel circPIP5K1A/miR-600/HIF-1alpha axis, which provides 
      candidate markers and therapeutic targets for NSCLC.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Chi, Yongbing
AU  - Chi Y
AD  - Clinical Laboratory, Shanghai Pudong New Area Gongli Hospital, Shanghai, China.
FAU - Luo, Qiancheng
AU  - Luo Q
AD  - Department of Emergency Medicine, Shanghai Pudong New Area Gongli Hospital, The 
      Second Military Medical University, Shanghai, China.
FAU - Song, Yuting
AU  - Song Y
AD  - Ningxia Medical University, Ningxia, China.
AD  - Sino-French Cooperative Central Lab, Shanghai Gongli Hospital, Secondary Military 
      Medical University, Shanghai, China.
FAU - Yang, Fangsong
AU  - Yang F
AD  - Anhui Gaolu Winery Staff Hospital, Bozhou, Shanghai, China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Central Laboratory, Shanghai Gongli Hospital, The Second Military 
      Medical University, Shanghai, China.
FAU - Jin, Mingming
AU  - Jin M
AUID- ORCID: 0000-0003-3574-2410
AD  - Department of Central Laboratory, Shanghai Gongli Hospital, The Second Military 
      Medical University, Shanghai, China.
FAU - Zhang, Denghai
AU  - Zhang D
AD  - Department of Central Laboratory, Shanghai Gongli Hospital, The Second Military 
      Medical University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190626
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (MIRN600 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Non-Small-Cell Lung/genetics/*metabolism/pathology
MH  - *Cell Proliferation
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/*metabolism
MH  - Lung Neoplasms/genetics/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - RNA, Circular/genetics/*metabolism
MH  - RNA, Neoplasm/genetics/*metabolism
OTO - NOTNLM
OT  - circPIP5K1A
OT  - hypoxia-inducible factor-1alpha
OT  - miR-600
OT  - non-small cell lung cancer
OT  - proliferation and metastasis
EDAT- 2019/06/27 06:00
MHDA- 2020/09/09 06:00
CRDT- 2019/06/27 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/27 06:00 [pubmed]
PHST- 2020/09/09 06:00 [medline]
PHST- 2019/06/27 06:00 [entrez]
AID - 10.1002/jcb.29225 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Nov;120(11):19019-19030. doi: 10.1002/jcb.29225. Epub 2019 
      Jun 26.