PMID- 31241700 OWN - NLM STAT- MEDLINE DCOM- 20190726 LR - 20190726 IS - 1678-2690 (Electronic) IS - 0001-3765 (Linking) VI - 91 IP - 2 DP - 2019 Jun 19 TI - Physiological parameters of Piaractus mesopotamicus (Osteichthyes: Characidae) exposed to a biopesticide based on Bacillus thuringiensis. PG - e20180474 LID - S0001-37652019000300612 [pii] LID - 10.1590/0001-3765201920180474 [doi] AB - This study investigated the physiological effects of a pesticide based on Bacillus thuringiensis (Dipel-WP(R)) added in the water and diet of Piaractus mesopotamicus during 24 and 48 h. It was added 0.13 g of de B. thuringiensis per kg of commercial feed; and for the fish subjected to the biopesticide in the water of the tanks, it was added 0.13 g/L of the biopesticide. Plasma levels of sodium, chloride, potassium, cholesterol, glucose, triglycerides, cortisol, total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST), hematocrit, hemoglobin, erythrocytes number, mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), number of thrombocytes and leukocytes were determined. Cortisol, lactate, glucose, total protein, cholesterol, triglycerides, ALT, AST, sodium, potassium, hematocrit, hemoglobin, MCV, number of erythrocytes, leukocytes, lymphocytes, eosinophils and PAS-positive granular leukocytes suffered alterations derived from the addition of B. thuringiensis in water and diet of the fish. The toxicity of the concentrations of biopesticide in short-term exposure in water and diet of the fish led to blood alterations (increase or decrease). Therefore, care must be taken to avoid a possible prolonged contamination in the tanks of fish farming by agricultural pollution based on B. thuringiensis. FAU - Mariano, Wagner S AU - Mariano WS AD - Programa de Pos-Graduacao em Biodiversidade e Biotecnologia/BIONORTE, Universidade Federal do Amapa/UNIFAP, Rodovia Juscelino Kubitschek, Km 2, 68903-419 Macapa, AP, Brazil. AD - Universidade Federal do Tocantins/UFT, Campus Universitario de Araguaina, Avenida Paraguai, s/n, Setor Cimba, 77824-838 Araguaina, TO, Brazil. FAU - Azevedo, Saulo B AU - Azevedo SB AD - Programa de Pos-Graduacao em Zoologia, Museu Paraense Emilio Goeldi, Avenida Governador Magalhaes Barata, 376, 66040-170 Belem, PA, Brazil. FAU - Gomes, Francisco L AU - Gomes FL AD - Universidade Federal do Tocantins/UFT, Campus Universitario de Araguaina, Avenida Paraguai, s/n, Setor Cimba, 77824-838 Araguaina, TO, Brazil. FAU - Lima, Liana B D AU - Lima LBD AD - Universidade Federal do Tocantins/UFT, Campus Universitario de Araguaina, Avenida Paraguai, s/n, Setor Cimba, 77824-838 Araguaina, TO, Brazil. FAU - Moron, Sandro E AU - Moron SE AD - Universidade Federal do Tocantins/UFT, Campus Universitario de Araguaina, Avenida Paraguai, s/n, Setor Cimba, 77824-838 Araguaina, TO, Brazil. FAU - Tavares-Dias, Marcos AU - Tavares-Dias M AUID- ORCID: 0000-0002-8376-1846 AD - Programa de Pos-Graduacao em Biodiversidade e Biotecnologia/BIONORTE, Universidade Federal do Amapa/UNIFAP, Rodovia Juscelino Kubitschek, Km 2, 68903-419 Macapa, AP, Brazil. AD - Embrapa Amapa, Rodovia Juscelino Kubitschek, Km 6, 68903-419 Macapa, AP, Brazil. LA - eng PT - Journal Article DEP - 20190619 PL - Brazil TA - An Acad Bras Cienc JT - Anais da Academia Brasileira de Ciencias JID - 7503280 RN - 0 (Pesticides) RN - 0 (Water Pollutants, Chemical) MH - Animals MH - Bacillus thuringiensis/*chemistry MH - Characidae/*blood/classification MH - Pesticides/*pharmacology MH - Time Factors MH - Water Pollutants, Chemical/*pharmacology EDAT- 2019/06/27 06:00 MHDA- 2019/07/28 06:00 CRDT- 2019/06/27 06:00 PHST- 2018/05/15 00:00 [received] PHST- 2018/09/10 00:00 [accepted] PHST- 2019/06/27 06:00 [entrez] PHST- 2019/06/27 06:00 [pubmed] PHST- 2019/07/28 06:00 [medline] AID - S0001-37652019000300612 [pii] AID - 10.1590/0001-3765201920180474 [doi] PST - epublish SO - An Acad Bras Cienc. 2019 Jun 19;91(2):e20180474. doi: 10.1590/0001-3765201920180474.