PMID- 31243722
OWN - NLM
STAT- MEDLINE
DCOM- 20191121
LR  - 20200626
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 50
IP  - 3
DP  - 2019 Jul
TI  - Increased serum ferritin and interleukin-18 levels in children with dengue.
PG  - 649-656
LID - 10.1007/s42770-019-00105-2 [doi]
AB  - Activated monocytes/macrophages that produce a cytokine storm play an important 
      role in the pathogenesis of dengue. Interleukin-18 (IL-18) is a proinflammatory 
      cytokine produced by monocyte/macrophages that is increased during dengue. 
      Ferritin is an acute-phase reactant and expressed by cells of the 
      reticulo-endothelial system in response to infection by dengue virus. The aims of 
      this study were to analyze the simultaneous expression of both IL-18 and 
      ferritins in children infected by diverse serotypes of dengue virus (DENV) and 
      determine their association with dengue severity. In this regard, children with 
      dengue (n = 25) and healthy controls with similar age and sex (n = 20) were 
      analyzed for circulating ferritin and cytokines. Monocytes were isolated by 
      Hystopaque gradient and co-cultured with DENV-2. IL-18 and ferritin contents in 
      blood, and IL-18 in culture supernatants were determined by ELISA. Increased 
      levels of ferritin and IL-18 (p < 0.0001) were observed in dengue patients, not 
      associated to NS1expression or type of infection (primary or secondary). Highest 
      values of both molecules (p < 0.001) were observed in dengue with warning signs 
      and severe dengue. Differential effect on IL-18/ferritin production was observed 
      associated to viral serotype infection. There were no correlations between 
      ferritin vs. IL-18 production, ferritin vs. NS1 status, and IL-18 vs. NS1 status. 
      Viral-infected monocyte cultures showed increased production of IL-18 
      (p < 0.001). In conclusion, increased circulating ferritin and IL-18 are 
      expressed in children infected by different serotypes of DENV associated with 
      dengue severity.
FAU - Valero, Nereida
AU  - Valero N
AD  - Instituto de Investigaciones Clinicas "Dr. Americo Negrette", Facultad de 
      Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Zulia, 4001-A, 
      Venezuela. valero.nereida@gmail.com.
AD  - Universidad Estatal del Sur de Manabi, Jipijapa, Ecuador. 
      valero.nereida@gmail.com.
FAU - Mosquera, Jesus
AU  - Mosquera J
AD  - Instituto de Investigaciones Clinicas "Dr. Americo Negrette", Facultad de 
      Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Zulia, 4001-A, 
      Venezuela.
FAU - Torres, Mariana
AU  - Torres M
AD  - Instituto de Investigaciones Clinicas "Dr. Americo Negrette", Facultad de 
      Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Zulia, 4001-A, 
      Venezuela.
FAU - Duran, Anyelo
AU  - Duran A
AD  - Instituto de Investigaciones Clinicas "Dr. Americo Negrette", Facultad de 
      Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Zulia, 4001-A, 
      Venezuela.
FAU - Velastegui, Maria
AU  - Velastegui M
AD  - Instituto de Investigaciones Clinicas "Dr. Americo Negrette", Facultad de 
      Medicina, Universidad del Zulia, Apartado Postal 23, Maracaibo, Zulia, 4001-A, 
      Venezuela.
FAU - Reyes, Javier
AU  - Reyes J
AD  - Universidad Estatal del Sur de Manabi, Jipijapa, Ecuador.
FAU - Fernandez, Miriam
AU  - Fernandez M
AD  - Facultad Ciencias de la Salud, Universidad Tecnica de Ambato, Ambato, Ecuador.
FAU - Fernandez, Gerardo
AU  - Fernandez G
AD  - Facultad Ciencias de la Salud, Universidad Tecnica de Ambato, Ambato, Ecuador.
FAU - Veliz, Teresa
AU  - Veliz T
AD  - Universidad Estatal del Sur de Manabi, Jipijapa, Ecuador.
LA  - eng
PT  - Journal Article
DEP - 20190626
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-18)
RN  - 9007-73-2 (Ferritins)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/metabolism
MH  - Dengue/*blood/diagnosis/metabolism/virology
MH  - Dengue Virus/classification/genetics/isolation & purification/physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Ferritins/*blood
MH  - Humans
MH  - Infant
MH  - Interleukin-18/*blood
MH  - Macrophages/metabolism
MH  - Male
MH  - Monocytes/metabolism
MH  - Serogroup
MH  - Severity of Illness Index
PMC - PMC6863311
OTO - NOTNLM
OT  - Children
OT  - Dengue
OT  - Ferritin
OT  - IL-18
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/06/28 06:00
MHDA- 2019/11/22 06:00
PMCR- 2020/06/26
CRDT- 2019/06/28 06:00
PHST- 2019/01/10 00:00 [received]
PHST- 2019/06/12 00:00 [accepted]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/11/22 06:00 [medline]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2020/06/26 00:00 [pmc-release]
AID - 10.1007/s42770-019-00105-2 [pii]
AID - 105 [pii]
AID - 10.1007/s42770-019-00105-2 [doi]
PST - ppublish
SO  - Braz J Microbiol. 2019 Jul;50(3):649-656. doi: 10.1007/s42770-019-00105-2. Epub 
      2019 Jun 26.