PMID- 31244439
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20200831
IS  - 1875-5739 (Electronic)
IS  - 1567-2026 (Linking)
VI  - 16
IP  - 3
DP  - 2019
TI  - A Potential Role for the Existence of Pericytes in the Neurovascular Unit of the 
      Sexually Dimorphic Nucleus of the Rat Preoptic Area to Control Blood-Brain 
      Barrier Function.
PG  - 194-201
LID - 10.2174/1567202616666190627120135 [doi]
AB  - BACKGROUND: The present study aimed at determining pericytes, a missing component 
      in the previously proposed living neurovascular unit (NVU) of the sexually 
      dimorphic nucleus of the preoptic area (SDN-POA) in rats. MATERIALS AND METHODS: 
      Calbindin D28K-immunoreactivities (CB28-irs) were used to delineate the SDN-POA 
      in which CD13-immunoreactivities (CD13-irs) or alpha-smooth muscle 
      actinimmunoreactivities (alphaSMA-irs), two pericyte biomarkers serving the indexes 
      of pericytes, were tagged using two adjacent brain sections (90-micron 
      intervals). In addition, the nestinimmunoreactive (nestin-ir) cells in the 
      SDN-POA were counted as pericytes referring to additional standards: location and 
      nucleic and cellular morphology. Male SDN-POA volume (5.0+/-0.3x10-3 mm3) was 
      significantly larger than the female (1.7+/-0.3x10-3 mm3). Within the SDN-POA, the 
      CD13-irs were characterized as dots, densely packed and net-like in distribution, 
      while the alphaSMAirs, excluding pipe-like or circular structures, appeared as short 
      rod-like structures that were sparsely distributed. RESULTS: The immunoreactive 
      counts of alpha-smooth muscle actin were 353+/-57/mm2 in males and 124+/-46/mm2 in 
      females (p<0.05). On the other hand, densities of the dot-like CD13-irs were 
      similar between males (4009+/-301/mm2) and females (4018+/-414/ mm2). There was no 
      difference between the male and the female in the nestin-ir pericyte count in the 
      SDN-POA. CONCLUSION: In conclusion, the present study adds new information 
      concerning pericytes to the living NVU of the SDN-POA. There is a difference of 
      sex in the count of the alphaSMA-irs in the living NVU of the SDN-POA. However, why 
      such a difference exists warrants further investigations.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - He, Zhen
AU  - He Z
AD  - Department of Neuroscience, HFT-132, National Center for Toxicological Research, 
      Food and Drug Administration, 3900 NCTR Road, Jefferson AR 72079, United States.
FAU - Patterson, Tucker A
AU  - Patterson TA
AD  - Office of the Director National Center for Toxicological Research, Food and Drug 
      Administration, Jefferson, AR 72079, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United Arab Emirates
TA  - Curr Neurovasc Res
JT  - Current neurovascular research
JID - 101208439
SB  - IM
MH  - Animals
MH  - Blood-Brain Barrier/*cytology/*physiology
MH  - Cell Count/methods
MH  - Female
MH  - Male
MH  - Pericytes/*physiology
MH  - Preoptic Area/*cytology/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Sex Characteristics
OTO - NOTNLM
OT  - Alpha-smooth muscle actin
OT  - CD13-immunoreactivity
OT  - living neurovascular unit
OT  - nestin
OT  - pericyte
OT  - sexually dimorphic nucleus.
EDAT- 2019/06/28 06:00
MHDA- 2020/09/01 06:00
CRDT- 2019/06/28 06:00
PHST- 2019/04/23 00:00 [received]
PHST- 2019/04/29 00:00 [revised]
PHST- 2019/05/01 00:00 [accepted]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2019/06/28 06:00 [entrez]
AID - CNR-EPUB-99250 [pii]
AID - 10.2174/1567202616666190627120135 [doi]
PST - ppublish
SO  - Curr Neurovasc Res. 2019;16(3):194-201. doi: 10.2174/1567202616666190627120135.