PMID- 31244611
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 13
DP  - 2019
TI  - Co-transplantation of Epidermal Neural Crest Stem Cells and Olfactory Ensheathing 
      Cells Repairs Sciatic Nerve Defects in Rats.
PG  - 253
LID - 10.3389/fncel.2019.00253 [doi]
LID - 253
AB  - Cell-based therapy is an alternative strategy to improve outcomes of peripheral 
      nerve injury (PNI). Epidermal neural crest stem cell (EPI-NCSC) is obtained from 
      autologous tissue without immunological rejection, which could expand quickly in 
      vitro and is suitable candidate for cell-based therapy. Olfactory ensheathing 
      cell (OEC) could secrete multiple neurotrophic factors (NTFs), which is often 
      used to repair PNI individually. However, whether the combination of EPI-NCSC and 
      OEC have better effects on PNI repair remains unclear. Here we use EPI-NCSC and 
      OEC co-transplantation in a rat sciatic nerve defect model to ascertain the 
      effects and potential mechanisms of cells co-transplantation on PNI. The effect 
      of EPI-NCSC and OEC co-transplantation on PNI is assessed by using a combination 
      of immunohistochemistry (IHC), electrophysiological recording and neural function 
      test. Co-transplantation of EPI-NCSC and OEC exerts a beneficial effect upon PNI 
      such as better organized structure, nerve function recovery, and lower motoneuron 
      apoptosis. IHC and enzyme-linked immuno sorbent assay (ELISA) further demonstrate 
      that cells co-transplantation may improve PNI via the expression of brain derived 
      growth factor (BDNF) and nerve growth factor (NGF) up-regulated by EPI-NCSC and 
      OEC synergistically. Eventually, the results from this study reveal that EPI-NCSC 
      and OEC co-transplantation effectively repairs PNI through enhancing the level of 
      BDNF and NGF, indicating that cells co-transplantation may serve as a fruitful 
      avenue for PNI in clinic treatment.
FAU - Zhang, Lu
AU  - Zhang L
AD  - Department of Pediatric Research Institute, Children's Hospital of Chongqing 
      Medical University, Ministry of Education Key Laboratory of Child Development and 
      Disorders, China International Science and Technology Cooperation Base of Child 
      Development and Critical Disorders, Chongqing Key Laboratory of Translational 
      Medical Research in Cognitive Development and Learning and Memory Disorders, 
      Chongqing, China.
FAU - Li, Bingcang
AU  - Li B
AD  - State Key Laboratory of Trauma, Burns and Combined Injury, Daping Hospital, 
      Research Institute of Surgery, Third Military Medical University, Chongqing, 
      China.
FAU - Liu, Bin
AU  - Liu B
AD  - Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of 
      Education, School of Life Sciences, Southwest University, Chongqing, China.
FAU - Dong, Zhifang
AU  - Dong Z
AD  - Department of Pediatric Research Institute, Children's Hospital of Chongqing 
      Medical University, Ministry of Education Key Laboratory of Child Development and 
      Disorders, China International Science and Technology Cooperation Base of Child 
      Development and Critical Disorders, Chongqing Key Laboratory of Translational 
      Medical Research in Cognitive Development and Learning and Memory Disorders, 
      Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20190612
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC6582070
OTO - NOTNLM
OT  - brain derived growth factor
OT  - co-transplantation
OT  - epidermal neural crest stem cell
OT  - nerve growth factor
OT  - olfactory ensheathing cell
OT  - peripheral nerve injury
EDAT- 2019/06/28 06:00
MHDA- 2019/06/28 06:01
PMCR- 2019/01/01
CRDT- 2019/06/28 06:00
PHST- 2019/02/20 00:00 [received]
PHST- 2019/05/21 00:00 [accepted]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/06/28 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2019.00253 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2019 Jun 12;13:253. doi: 10.3389/fncel.2019.00253. 
      eCollection 2019.