PMID- 31244665
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 10
DP  - 2019
TI  - Curcumin/Liposome Nanotechnology as Delivery Platform for Anti-inflammatory 
      Activities via NFkB/ERK/pERK Pathway in Human Dental Pulp Treated With 
      2-HydroxyEthyl MethAcrylate (HEMA).
PG  - 633
LID - 10.3389/fphys.2019.00633 [doi]
LID - 633
AB  - Curcumin, primary component of the spice turmeric extracted from the rhizomes of 
      Curcuma longa, represents the major anti-oxidant and anti-inflammatory substance 
      found in turmeric, acting thought various mechanisms not completely understood. 
      Curcumin modulates cytokines, growth factors, transcription factors, inflammatory 
      molecules and cell signaling pathways. During restorative dentistry practice, 
      free resin monomers of 2-hydroxyethyl methacrylate (HEMA) propagate through 
      dentin micro-channel and pulp into the bloodstream affecting cellular integrity. 
      The study highlights the significance of application of curcumin bioactive 
      component into liposomal formulations (CurLIP) to restore the homeostasis of 
      dental pulp stem cells (hDPSCs) in response to 3 and 5 mmol L(-1) HEMA treatment. 
      Cell proliferation in combination with changes of the morphological features, 
      proinflammatory cytokines secretion as Interleukin (IL) 6, IL8, Monocyte 
      Chemoattractant Protein-1 (MCP1) and Interferon-gamma (IFNgamma) were assayed along 
      with the nuclear factor (NF)-kB, an inducible transcription factor involved in 
      the activation of several cell processes associated to extracellular 
      signal-regulated kinases (ERK) and posphorylated (p-) ERK pathway. Our results 
      showed a decreased cell proliferation, morphological changes and upregulation of 
      IL6, IL8, MCP1 and IFNgamma in presence of 3 and 5 mmol L(-1) HEMA treatment. CurLIP 
      therapy in hDPSCs provokes an increase in cell proliferation and the block of 
      inflammatory cytokines secretion through the inhibitory regulation of NFkB/ERK 
      and pERK signaling cascade. The natural nanocarrier CurLIP influences numerous 
      biochemical and molecular cascades causing anti-inflammatory properties in 
      response to HEMA treatment in human dental pulp stem cells, representing an 
      innovative endodontic formulation able to improve the quality of dental care with 
      a major human community impact.
FAU - Sinjari, Bruna
AU  - Sinjari B
AD  - Department of Medical Oral and Biotechnological Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
FAU - Pizzicannella, Jacopo
AU  - Pizzicannella J
AD  - Department of Medical Oral and Biotechnological Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
AD  - ASL02 Lanciano-Vasto-Chieti, "Ss. Annunziata" Hospital, Chieti, Italy.
FAU - D'Aurora, Marco
AU  - D'Aurora M
AD  - Department of Psychological, Health and Territorial Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
FAU - Zappacosta, Romina
AU  - Zappacosta R
AD  - Department of Pharmacy, University "G. d'Annunzio", Chieti, Italy.
FAU - Gatta, Valentina
AU  - Gatta V
AD  - Department of Psychological, Health and Territorial Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
FAU - Fontana, Antonella
AU  - Fontana A
AD  - Department of Pharmacy, University "G. d'Annunzio", Chieti, Italy.
FAU - Trubiani, Oriana
AU  - Trubiani O
AD  - Department of Medical Oral and Biotechnological Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
FAU - Diomede, Francesca
AU  - Diomede F
AD  - Department of Medical Oral and Biotechnological Sciences, University "G. 
      d'Annunzio", Chieti, Italy.
LA  - eng
PT  - Journal Article
DEP - 20190611
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC6579913
OTO - NOTNLM
OT  - NFkB/ERK/pERK
OT  - human dental pulp stem cells
OT  - inflammation
OT  - liposome curcumin
OT  - methacrylate
EDAT- 2019/06/28 06:00
MHDA- 2019/06/28 06:01
PMCR- 2019/06/11
CRDT- 2019/06/28 06:00
PHST- 2019/01/31 00:00 [received]
PHST- 2019/05/06 00:00 [accepted]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/06/28 06:01 [medline]
PHST- 2019/06/11 00:00 [pmc-release]
AID - 10.3389/fphys.2019.00633 [doi]
PST - epublish
SO  - Front Physiol. 2019 Jun 11;10:633. doi: 10.3389/fphys.2019.00633. eCollection 
      2019.