PMID- 31245758
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231011
IS  - 2475-4455 (Electronic)
IS  - 2475-4455 (Linking)
VI  - 3
IP  - 2
DP  - 2019 Feb
TI  - A new insight to explore the regulation between S-nitrosylation and 
      N-glycosylation.
PG  - e00110
LID - 10.1002/pld3.110 [doi]
LID - e00110
AB  - Nitric oxide (NO) is a signal molecule in plants and animals. Arabidopsis GSNO 
      reductase1 (AtGSNOR1) catalyzes metabolism of S-nitrosoglutathione (GSNO) which 
      is a major biologically active NO species. The GSNOR1 loss-of-function mutant 
      gsnor1-3 overaccumulates GSNO with inherent high S-nitrosylation level and 
      resistance to the oxidative stress inducer paraquat 
      (1,1'-dimethyl-4,4'-bipyridinium dichloride). Here, we report the 
      characterization of dgl1-3 as a genetic suppressor of gsnor1-3. DGL1 encodes a 
      subunit of the oligosaccharyltransferse (OST) complex which catalyzes the 
      formation of N-glycosidic bonds in N-glycosylation. The fact that dgl1-3 
      repressed the paraquat resistance of gsnor1-3 meanwhile gsnor1-3 rescued the 
      embryo-lethal and post-embryonic development defect of dgl1-3 reminded us the 
      possibility that S-nitrosylation and N-glycosylation crosstalk with each other 
      through co-substrates. By enriching glycoproteins in gsnor1-3 and mass 
      spectrometry analysis, TGG2 (thioglucoside glucohydrolase2) was identified as one 
      of co-substrates with high degradation rate and elevated N-glycosylation level in 
      gsnor1-3 ost3/6. The S-nitrosylation and N-glycosylation profiles were also 
      modified in dgl1-3 and gsnor1-3. Thereby, we propose a linkage between 
      S-nitrosylation and N-glycosylation through co-substrates.
FAU - Du, Hu
AU  - Du H
AD  - Vegetable Research Institute Guangdong Academy of Agricultural Sciences Guangdong 
      Key Laboratory for New Technology Research of Vegetables Guangzhou China.
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
AD  - University of Chinese Academy of Sciences Beijing China.
FAU - Chen, Lichao
AU  - Chen L
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
AD  - University of Chinese Academy of Sciences Beijing China.
FAU - Zhan, Ni
AU  - Zhan N
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
AD  - University of Chinese Academy of Sciences Beijing China.
FAU - Mu, Jinye
AU  - Mu J
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
FAU - Ren, Bo
AU  - Ren B
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
FAU - Zuo, Jianru
AU  - Zuo J
AD  - State Key Laboratory of Plant Genomics and National Plant Gene Research Center 
      (Beijing) Institute of Genetics and Developmental Biology Chinese Academy of 
      Sciences Beijing China.
LA  - eng
PT  - Journal Article
DEP - 20190301
PL  - England
TA  - Plant Direct
JT  - Plant direct
JID - 101716131
PMC - PMC6508853
OTO - NOTNLM
OT  - Arabidopsis
OT  - DGL1
OT  - N-glycosylation
OT  - S-nitrosylation
OT  - TGG2
OT  - gsnor1-3
EDAT- 2019/06/28 06:00
MHDA- 2019/06/28 06:01
PMCR- 2019/03/01
CRDT- 2019/06/28 06:00
PHST- 2018/11/11 00:00 [received]
PHST- 2018/12/11 00:00 [revised]
PHST- 2018/12/13 00:00 [accepted]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/06/28 06:01 [medline]
PHST- 2019/03/01 00:00 [pmc-release]
AID - PLD3110 [pii]
AID - 10.1002/pld3.110 [doi]
PST - epublish
SO  - Plant Direct. 2019 Mar 1;3(2):e00110. doi: 10.1002/pld3.110. eCollection 2019 
      Feb.