PMID- 31245956
OWN - NLM
STAT- MEDLINE
DCOM- 20190905
LR  - 20190906
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 35
IP  - 1
DP  - 2019 Jan
TI  - [Effects of dexmedetomidine on the growth and development of rat hippocampal 
      neurons and its mechanism].
PG  - 69-73
AB  - OBJECTIVE: To investigate the effects of dexmedetomidine (DEX) on hippocampal 
      neuron development process and the expressions of molecules in brain-derived 
      neurotropic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling pathway in 
      neonatal rats. METHODS: The hippocampal neurons were isolated from neonatal rats 
      and cultured in vitro. The cells were seeded in 96-well plates,which were divided 
      into 4 groups (control group, 1 mumol/L DEX treatment group, 5 mumol/L DEX 
      treatment group, 50 mumol/L DEX treatment group), six wells were set in each 
      group, and different concentrations of dexmedetomidine 1, 5 and 50 mumol/L were 
      administered respectively. Cell viability was detected at 2, 4, 6, 8, and 10 d 
      after treatment, and apoptosis was detected 10 days after treatment. The mRNA 
      expression levels of synaptophysin (SYN) and postsynaptic density protein 95 
      (PSD95) were detected by q-PCR, and the expressions of BDNF, TrkB and 
      N-methyl-D-aspartate receptor (NMDAR) protein were analyzed. RESULTS: Compared 
      with the control group, there was no significant difference in neuronal cell 
      viability between the different doses of DEX treatment group. There was no 
      significant difference in the expression of SYN and PSD95 mRNA and TrkB protein 
      between the 1 mumol/L and 5 mumol/L DEX treatment groups (P＞0.05). The expression 
      levels of SYN and PSD95 mRNA in the 50 mumol/L DEX group were increased 
      significantly (P＜0.01), and the expression level of BDNF protein was up-regulated 
      significantly (P＜0.01), the expression of the p-N-methyl-D-aspartate receptor was 
      increased (P＜0.01). CONCLUSION: 50 mumol/L DEX has a certain effect on rat 
      hippocampal neurons, which may be achieved by up-regulating the expression of 
      BDNF and the phosphorylation level of N-methyl-D-aspartate receptor.
FAU - Liu, Yu-Xin
AU  - Liu YX
AD  - Department of Anesthesiology, Yongchuan District Children's Hospital, Chongqing 
      402160.
FAU - Yan, Dong
AU  - Yan D
AD  - Yongchuan Hospital Affiliated to Medical University of Chongqing, Chongqing 
      402160, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Hypnotics and Sedatives)
RN  - 67VB76HONO (Dexmedetomidine)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - *Dexmedetomidine/pharmacology
MH  - Growth and Development
MH  - *Hippocampus/drug effects/growth & development
MH  - *Hypnotics and Sedatives/pharmacology
MH  - *Neurons/drug effects
MH  - Rats
EDAT- 2019/06/28 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/06/28 06:00
PHST- 2019/06/28 06:00 [entrez]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
AID - 10.12047/j.cjap.5715.2019.017 [doi]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2019 Jan;35(1):69-73. doi: 
      10.12047/j.cjap.5715.2019.017.