PMID- 31247472
OWN - NLM
STAT- MEDLINE
DCOM- 20191122
LR  - 20191122
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 125
DP  - 2019 Aug
TI  - Development of canine X-chromosome inactivation pattern analysis for the 
      detection of cell clonality by incorporating the examination of the SLIT and 
      NTRK-like family member 4 (SLITRK4) gene.
PG  - 170-175
LID - S0034-5288(18)35384-0 [pii]
LID - 10.1016/j.rvsc.2019.06.004 [doi]
AB  - X-chromosome inactivation pattern (XCIP) analysis can be used to assess the 
      clonality of cell populations of various origin by distinguishing the methylated 
      X chromosome from the unmethylated X chromosome. In this study, the utility of 
      XCIP analysis was improved by incorporating the examination of AC dinucleotide 
      repeats in SLIT and NTRK-like family member 4 (SLITRK4) gene into the previously 
      reported CAG repeat examination of androgen receptor (AR) gene in dogs. The rate 
      of heterozygosity when both genes were analysed (125/150, 83.3%) was higher than 
      AR gene examination alone (86/150, 57.3%). Blood samples from heterozygous dogs 
      in either AC-1 or AC-2 of SLITRK4 gene were examined for the corrected 
      inactivation allele ratio (CIAR), resulting in the determination of a reference 
      range of CIAR <3.8 in non-neoplastic cell/tissue samples. Using this analytical 
      method, 49% (21/43) of neoplastic tissue samples from dogs showed a CIAR >3.8, 
      indicating the presence of a clonal population. Through the present study, the 
      availability of canine XCIP analysis was improved by incorporating the 
      examination of the SLITRK4 gene, providing a highly useful laboratory examination 
      system for the detection of the clonality of various cell/tissue samples in dogs.
CI  - Copyright (c) 2019. Published by Elsevier Ltd.
FAU - Tomita, A
AU  - Tomita A
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 
      Japan.
FAU - Mochizuki, H
AU  - Mochizuki H
AD  - Department of Molecular Biomedical Sciences, College of Veterinary Medicine, 
      North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27607, 
      USA.
FAU - Tsuboi, M
AU  - Tsuboi M
AD  - Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, the 
      University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Ogura, I
AU  - Ogura I
AD  - KOJIMA Animal Hospital, KOJIMA Co., Ltd., 3-60-21 Kameido, Koto-ku, Tokyo 
      136-8510, Japan.
FAU - Igarashi, H
AU  - Igarashi H
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 
      Japan.
FAU - Goto-Koshino, Y
AU  - Goto-Koshino Y
AD  - Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, the 
      University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Takahashi, M
AU  - Takahashi M
AD  - Laboratory of Small Animal Internal Medicine, Joint Faculty of Veterinary 
      Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima-shi, Kagoshima 
      890-0065, Japan.
FAU - Ohmi, A
AU  - Ohmi A
AD  - Veterinary Medical Center, Graduate School of Agricultural and Life Sciences, the 
      University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Tomiyasu, H
AU  - Tomiyasu H
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 
      Japan.
FAU - Ohno, K
AU  - Ohno K
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 
      Japan.
FAU - Nakagawa, T
AU  - Nakagawa T
AD  - Department of Veterinary Surgery, Graduate School of Agricultural and Life 
      Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Uchida, K
AU  - Uchida K
AD  - Department of Veterinary Pathology, Graduate School of Agricultural and Life 
      Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Nishimura, R
AU  - Nishimura R
AD  - Department of Veterinary Surgery, Graduate School of Agricultural and Life 
      Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
FAU - Tsujimoto, H
AU  - Tsujimoto H
AD  - Department of Veterinary Internal Medicine, Graduate School of Agricultural and 
      Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 
      Japan. Electronic address: atsuji@mail.ecc.u-tokyo.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20190611
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Androgen)
SB  - IM
MH  - Alleles
MH  - Animals
MH  - Cell Lineage
MH  - Dog Diseases/genetics/metabolism
MH  - Dogs
MH  - Female
MH  - Gene Expression Regulation
MH  - Heterozygote
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Neoplasms/genetics/metabolism
MH  - Receptors, Androgen/genetics/*metabolism
MH  - X Chromosome/*physiology
MH  - *X Chromosome Inactivation
OTO - NOTNLM
OT  - Androgen receptor
OT  - Clonality
OT  - Dog
OT  - SLITRK4
OT  - XCIP
EDAT- 2019/06/28 06:00
MHDA- 2019/11/23 06:00
CRDT- 2019/06/28 06:00
PHST- 2018/10/27 00:00 [received]
PHST- 2019/06/09 00:00 [revised]
PHST- 2019/06/10 00:00 [accepted]
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2019/11/23 06:00 [medline]
PHST- 2019/06/28 06:00 [entrez]
AID - S0034-5288(18)35384-0 [pii]
AID - 10.1016/j.rvsc.2019.06.004 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Aug;125:170-175. doi: 10.1016/j.rvsc.2019.06.004. Epub 2019 Jun 
      11.