PMID- 31247598
OWN - NLM
STAT- MEDLINE
DCOM- 20200129
LR  - 20200802
IS  - 1758-5090 (Electronic)
IS  - 1758-5082 (Print)
IS  - 1758-5082 (Linking)
VI  - 11
IP  - 4
DP  - 2019 Aug 1
TI  - Neural layer self-assembly in geometrically confined rat and human 3D cultures.
PG  - 045011
LID - 10.1088/1758-5090/ab2d3f [doi]
AB  - Neurological disorders affect millions of Americans and this number is expected 
      to rise with the aging population. Development of drugs to treat these disorders 
      may be facilitated by improved in vitro models that faithfully reproduce salient 
      features of the relevant brain regions. Current 3D culture methods face 
      challenges with reliably reproducing microarchitectural features of brain 
      morphology such as cortical or hippocampal layers. In this work, 
      polydimethylsiloxane (PDMS) mini-wells were used to create low aspect ratio, 
      adherent 3D constructs where neurons self-assemble into layers. Layer 
      self-assembly was determined to depend on the size of the PDMS mini-well. Layer 
      formation occurred in cultures composed of primary rat cortical neurons or human 
      induced pluripotent stem cell-derived neurons and astrocytes and was robust and 
      reproducible. Layered 3D constructs were found to have spontaneous neural 
      activity characterized by long bursts similar to activity in the developing 
      cortex. The responses of layered 3D cultures to drugs were more similar to in 
      vivo data than those of 2D cultures. 3D constructs created with this method may 
      be thus suitable as in vitro models for drug discovery for neurological 
      disorders.
FAU - Hasan, Md Fayad
AU  - Hasan MF
AD  - Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, 
      PA 18015, United States of America.
FAU - Ghiasvand, Shabnam
AU  - Ghiasvand S
FAU - Wang, Huaixing
AU  - Wang H
FAU - Miwa, Julie M
AU  - Miwa JM
FAU - Berdichevsky, Yevgeny
AU  - Berdichevsky Y
LA  - eng
GR  - R21 NS088358/NS/NINDS NIH HHS/United States
GR  - R33 NS088358/NS/NINDS NIH HHS/United States
GR  - R44 DA032464/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190801
PL  - England
TA  - Biofabrication
JT  - Biofabrication
JID - 101521964
RN  - 4368-28-9 (Tetrodotoxin)
RN  - H030S2S85J (Kynurenic Acid)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Excitatory Postsynaptic Potentials/drug effects
MH  - Humans
MH  - Induced Pluripotent Stem Cells/cytology/drug effects
MH  - Kynurenic Acid/pharmacology
MH  - Neurons/*cytology/drug effects
MH  - Rats, Sprague-Dawley
MH  - Spheroids, Cellular/cytology/drug effects
MH  - Tetrodotoxin/pharmacology
PMC - PMC6908307
MID - NIHMS1061109
COIS- Conflicts of Interest The authors declare no conflict of interest.
EDAT- 2019/06/28 06:00
MHDA- 2020/01/30 06:00
PMCR- 2020/08/01
CRDT- 2019/06/28 06:00
PHST- 2019/06/28 06:00 [pubmed]
PHST- 2020/01/30 06:00 [medline]
PHST- 2019/06/28 06:00 [entrez]
PHST- 2020/08/01 00:00 [pmc-release]
AID - 10.1088/1758-5090/ab2d3f [doi]
PST - epublish
SO  - Biofabrication. 2019 Aug 1;11(4):045011. doi: 10.1088/1758-5090/ab2d3f.