PMID- 31247918
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 13
DP  - 2019 Jun 26
TI  - Isolated Compounds from Turpinia formosana Nakai Induce Ossification.
LID - 10.3390/ijms20133119 [doi]
LID - 3119
AB  - Bone metabolism is a homeostatic process, imbalance in which leads to the onset 
      of diseases such as osteoporosis and osteopenia. Although several drugs are 
      currently available to treat such conditions, they are associated with severe 
      side effects and do not enhance bone formation. Thus, identifying alternative 
      treatment strategies that focus on enhancing bone formation is essential. Herein, 
      we explored the osteogenic potential of Turpinia formosana Nakai using human 
      osteoblast (HOb) cells. The plant extract was subjected to various 
      chromatographic techniques to obtain six compounds, including one new compound: 
      3,3'-di-O-methylellagic acid-4-O-alpha-l-arabinofuranoside (1). Compounds 
      3,3'-di-O-methylellagic acid-4-O-alpha-l-arabinofuranoside (1), gentisic acid 
      5-O-beta-d-(6'-O-galloyl) glucopyranoside (2), strictinin (3), and 
      (-)-epicatechin-3-O-beta-d-allopyranoside (6) displayed no significant cytotoxicity 
      toward HOb cells, and thus their effects on various osteogenic markers were 
      analyzed. Results showed that 1-3 and 6 significantly increased alkaline 
      phosphatase (ALP) activity up to 120.0, 121.3, 116.4, and 125.1%, respectively. 
      Furthermore, 1, 2, and 6 also markedly enhanced the mineralization process with 
      respective values of up to 136.4, 118.9, and 134.6%. In addition, the new 
      compound, 1, significantly increased expression levels of estrogen receptor-alpha 
      (133.4%) and osteogenesis-related genes of Runt-related transcription factor 2 
      (Runx2), osteopontin (OPN), bone morphogenetic protein (BMP)-2, bone sialoprotein 
      (BSP), type I collagen (Col-1), and brain-derived neurotropic factor (BDNF) by at 
      least 1.5-fold. Our results demonstrated that compounds isolated from T. 
      formosana possess robust osteogenic potential, with the new compound, 1, also 
      exhibiting the potential to enhance the bone formation process. We suggest that 
      T. formosana and its isolated active compounds deserve further evaluation for 
      development as anti-osteoporotic agents.
FAU - Imtiyaz, Zuha
AU  - Imtiyaz Z
AD  - Clinical Drug Development of Herbal Medicine, Graduate Institute of 
      Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan.
FAU - Wang, Yi-Fang
AU  - Wang YF
AD  - Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical 
      University, Taipei 11031, Taiwan.
FAU - Lin, Yi-Tzu
AU  - Lin YT
AD  - Clinical Drug Development of Herbal Medicine, Graduate Institute of 
      Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan.
FAU - Liu, Hui-Kang
AU  - Liu HK
AD  - Clinical Drug Development of Herbal Medicine, Graduate Institute of 
      Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan.
AD  - Division of Basic Chinese Medicine, National Research Institute of Chinese 
      Medicine (NRICM), Ministry of Health and Welfare, Taipei 112, Taiwan.
FAU - Lee, Mei-Hsien
AU  - Lee MH
AUID- ORCID: 0000-0001-8592-9326
AD  - Clinical Drug Development of Herbal Medicine, Graduate Institute of 
      Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan. lmh@tmu.edu.tw.
AD  - Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical 
      University, Taipei 11031, Taiwan. lmh@tmu.edu.tw.
AD  - Center for Reproductive Medicine & Sciences, Taipei Medical University Hospital, 
      Taipei 11031, Taiwan. lmh@tmu.edu.tw.
LA  - eng
GR  - NSC101-2320-B-038-014-MY3(2-3)/Ministry of Science and Technology/
PT  - Journal Article
DEP - 20190626
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers)
RN  - 0 (Phytochemicals)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Biomarkers
MH  - Calcification, Physiologic/*drug effects
MH  - Gene Expression
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Structure
MH  - Osteoblasts/drug effects/metabolism
MH  - Osteogenesis/drug effects/genetics
MH  - Phytochemicals/*chemistry/isolation & purification/*pharmacology
MH  - Plant Extracts/*chemistry/isolation & purification/*pharmacology
MH  - Receptors, Estrogen/genetics/metabolism
MH  - Tracheophyta/*chemistry
PMC - PMC6651545
OTO - NOTNLM
OT  - 3,3'-di-O-methylellagic acid-4-O-alpha-l-arabinofuranoside
OT  - Turpinia formosana
OT  - alkaline phosphatase
OT  - bone formation
OT  - estrogen receptors
OT  - mineralization
OT  - osteoporosis
COIS- The authors declare no conflict of interest.
EDAT- 2019/06/30 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/07/01
CRDT- 2019/06/29 06:00
PHST- 2019/06/03 00:00 [received]
PHST- 2019/06/24 00:00 [revised]
PHST- 2019/06/24 00:00 [accepted]
PHST- 2019/06/29 06:00 [entrez]
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - ijms20133119 [pii]
AID - ijms-20-03119 [pii]
AID - 10.3390/ijms20133119 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Jun 26;20(13):3119. doi: 10.3390/ijms20133119.