PMID- 31251466
OWN - NLM
STAT- MEDLINE
DCOM- 20211102
LR  - 20211102
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 108
IP  - 3
DP  - 2020 Apr
TI  - Diopside-tricalcium phosphate bioactive ceramics for osteogenic differentiation 
      of human adipose stem cells.
PG  - 819-833
LID - 10.1002/jbm.b.34436 [doi]
AB  - Ti scaffolds combined with autologous human adipose-derived mesenchymal stem 
      cells (hASCs) have been successfully applied for regenerative 
      cranio-maxillofacial bone therapies. Future challenges reside in regeneration of 
      larger bone defects and displacement of the permanent Ti structure, thus, 
      advanced resorbable scaffolds are needed. Composites of beta-Ca(3) (PO(4) )(2) with 
      80 and 60 wt % of CaMg(SiO(3) )(2) with improved mechanical properties compared 
      to tricalcium phosphate (TCP) materials are presented. Synthetic CaMg(SiO(3) )(2) 
      and a precursor of Ca(3) (PO(4) )(2) were used to fabricate the composites and a 
      reference beta-Ca(3) (PO(4) )(2) material by uniaxial pressing and solid state 
      sintering. Optimum sintering temperature of 1225 degrees C was selected. Microstructural 
      analysis and Weibull distributions of tensile strengths determined by the 
      diametral compression of discs test are reported. Thermodynamic simulation of the 
      dissolution process in simulated body fluid body fluid was done. The biological 
      response with hASCs was analyzed using basic and osteogenic media. Viability and 
      osteogenic potential-LIVE/DEAD assay; alkaline phosphatase activity and collagen 
      type-I production-were characterized. The composites have higher tensile strength 
      (>3x) than TCP materials, for similar reliability, and support viability and 
      osteogenic differentiation of hASCs. Resorption of the high strength phase 
      diopside is the slowest. The promising results reported here suggest possible 
      uses of these bioactive beta-Ca(3) (PO(4) )(2) -CaMg(SiO(3) )(2) ceramics together 
      with hASCs in bone tissue engineering.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Vanhatupa, Sari
AU  - Vanhatupa S
AD  - Adult Stem Cell Group, Faculty of Medicine and Health Technology, Tampere 
      University, Tampere, Finland.
AD  - Research, Development and Innovation Centre, Tampere University Hospital, 
      Tampere, Finland.
FAU - Miettinen, Susanna
AU  - Miettinen S
AD  - Adult Stem Cell Group, Faculty of Medicine and Health Technology, Tampere 
      University, Tampere, Finland.
AD  - Research, Development and Innovation Centre, Tampere University Hospital, 
      Tampere, Finland.
FAU - Pena, Pilar
AU  - Pena P
AD  - Instituto de Ceramica y Vidrio, CSIC, Madrid, Spain.
FAU - Baudin, Carmen
AU  - Baudin C
AD  - Instituto de Ceramica y Vidrio, CSIC, Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190628
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Calcium Phosphates)
RN  - 1343-98-2 (Silicic Acid)
RN  - 14483-19-3 (diopside)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - K4C08XP666 (tricalcium phosphate)
SB  - IM
MH  - Adipocytes/*cytology
MH  - Alkaline Phosphatase/metabolism
MH  - Bone and Bones/pathology
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Humans
MH  - Kinetics
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Fluorescence
MH  - *Osteogenesis
MH  - Pressure
MH  - Reproducibility of Results
MH  - Silicic Acid/*chemistry
MH  - Stem Cells/*cytology
MH  - Stress, Mechanical
MH  - Temperature
MH  - Tensile Strength
MH  - Thermodynamics
MH  - Tissue Engineering/methods
MH  - X-Ray Diffraction
OTO - NOTNLM
OT  - calcium phosphates
OT  - cell differentiation
OT  - diopside
OT  - mechanical properties
OT  - stem cells
EDAT- 2019/06/30 06:00
MHDA- 2021/11/03 06:00
CRDT- 2019/06/29 06:00
PHST- 2019/01/24 00:00 [received]
PHST- 2019/05/11 00:00 [revised]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2019/06/29 06:00 [entrez]
AID - 10.1002/jbm.b.34436 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2020 Apr;108(3):819-833. doi: 
      10.1002/jbm.b.34436. Epub 2019 Jun 28.