PMID- 31251569
OWN - NLM
STAT- MEDLINE
DCOM- 20200916
LR  - 20200916
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 91
IP  - 15
DP  - 2019 Aug 6
TI  - Nondestructive Identification and Accurate Isolation of Single Cells through a 
      Chip with Raman Optical Tweezers.
PG  - 9932-9939
LID - 10.1021/acs.analchem.9b01604 [doi]
AB  - Raman optical tweezers (ROT) as a label-free technique plays an important role in 
      single-cell study such as heterogeneity of tumor and microbial cells. Herein we 
      designed a chip utilizing ROT to isolate a specific single cell. The chip was 
      made from a polydimethylsiloxane (PDMS) slab and formed into a gourd-shaped 
      reservoir with a connected channel on a cover glass. On the chip an individual 
      cell could be isolated from a cell crowd and then extracted with  approximately 0.5 muL of 
      phosphate-buffered saline (PBS) via pipet immediately after Raman spectral 
      measurements of the same cell. As verification, we separated four different type 
      of cells including BGC823 gastric cancer cells, erythrocytes, lymphocytes, and E. 
      coli cells and quantifiably characterized the heterogeneity of the cancer cells, 
      leukocyte subtype, and erythrocyte status, respectively. The average time of 
      identifying and isolating a specific cell was 3 min. Cell morphology comparison 
      and viability tests showed that the successful rate of single-cell isolation was 
      about 90%. Thus, we believe our platform could further couple other single-cell 
      techniques such as single-cell sequencing and become a multiperspective 
      analytical approach at the level of a single cell.
FAU - Fang, Teng
AU  - Fang T
AUID- ORCID: 0000-0003-2515-4180
FAU - Shang, Wenhao
AU  - Shang W
FAU - Liu, Chang
AU  - Liu C
FAU - Xu, Jingjing
AU  - Xu J
FAU - Zhao, Dongping
AU  - Zhao D
FAU - Liu, Yaoyao
AU  - Liu Y
FAU - Ye, Anpei
AU  - Ye A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190710
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Separation/instrumentation/*methods
MH  - Erythrocytes/cytology/physiology
MH  - Escherichia coli/isolation & purification
MH  - Humans
MH  - Lab-On-A-Chip Devices
MH  - Leukocytes/cytology/physiology
MH  - *Optical Tweezers
MH  - Single-Cell Analysis
MH  - Spectrum Analysis, Raman
EDAT- 2019/06/30 06:00
MHDA- 2020/09/17 06:00
CRDT- 2019/06/29 06:00
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2020/09/17 06:00 [medline]
PHST- 2019/06/29 06:00 [entrez]
AID - 10.1021/acs.analchem.9b01604 [doi]
PST - ppublish
SO  - Anal Chem. 2019 Aug 6;91(15):9932-9939. doi: 10.1021/acs.analchem.9b01604. Epub 
      2019 Jul 10.