PMID- 31251898
OWN - NLM
STAT- MEDLINE
DCOM- 20200225
LR  - 20200225
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 110
DP  - 2019 Oct
TI  - Comparison of multiple doses of cyclosporine A on germ cell apoptosis and 
      epididymal sperm parameters after testicular ischemia/reperfusion in rats.
PG  - 104271
LID - S0014-4800(18)30536-7 [pii]
LID - 10.1016/j.yexmp.2019.104271 [doi]
AB  - Testicular torsion/detorsion (T/D) is an inflammatory problem in men genital 
      system with infertility effects. Cyclosporine A (CsA) as an immunosuppressant 
      medication, exerts anti-inflammatory properties in tissue injuries. We sought to 
      compare the efficacy of 3 doses of CsA on oxidative stress, apoptosis and 
      epididymal sperm quality after ipsilateral testicular T/D. METHODS: 96 mature 
      male rats were divided into six groups 16 each in: Control group (Group1), Sham 
      operated (Group2), In rest groups, the right testis was twisted 720 degrees  in a 
      clockwise direction for 1 h; T/D + 0.1% dimethylsulfoxide) DMSO((Group3), and in 
      groups 4-6; CsA were administered 1, 5, and 10 mg/kg, intravenously (iv) 30 and 
      90 min after torsion, respectively. RESULTS: Tissue malondialdehyde (MDA) level 
      and caspase-3 activity increased and catalase (CAT), superoxide dismutase (SOD) 
      and glutathione peroxidase (GPx) activities decreased in compared with control 
      group 4 h after detorsion (p < .001). In six rats of each group 24 h after 
      detorsion, histopathological changes and germ cell apoptosis were significantly 
      deteriorated by determining mean of seminiferous tubules diameters (MSTD) and 
      TUNEL assay. Moreover, 30 days after T/D, sperm concentration and motility were 
      examined in rest of animals. CONCLUSIONS: Pre- and post-reperfusion CsA 
      diminished MDA and caspase-3levels and normalized antioxidant enzymes activities. 
      Germ cell apoptosis was significantly reduced, as well as, MSTD and long-term 
      sperm insults were improved. Inhibition of mitochondrial permeability transition 
      pore opening is suggested mechanism for cell protection against testicular T/D 
      insults.
CI  - Published by Elsevier Inc.
FAU - Yazdani, Iraj
AU  - Yazdani I
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Majdani, Raheleh
AU  - Majdani R
AD  - Department of Cellular and Molecular Biology, Faculty of Basic Science, 
      University of Maragheh, Maragheh, Iran.
FAU - Ghasemnejad-Berenji, Morteza
AU  - Ghasemnejad-Berenji M
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Urmia University 
      of Medical Sciences, Urmia, Iran.
FAU - Dehpour, Ahmad Reza
AU  - Dehpour AR
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran. Electronic address: dehpour@yahoo.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190625
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (Immunosuppressive Agents)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 83HN0GTJ6D (Cyclosporine)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Catalase/metabolism
MH  - Cyclosporine/*pharmacology
MH  - Epididymis/*drug effects/metabolism/pathology
MH  - Germ Cells
MH  - Glutathione Peroxidase/metabolism
MH  - Immunosuppressive Agents/pharmacology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/drug effects
MH  - Rats, Wistar
MH  - Reperfusion Injury/*physiopathology
MH  - Spermatic Cord Torsion/physiopathology
MH  - Spermatozoa/*drug effects/pathology
MH  - Superoxide Dismutase/metabolism
MH  - Testis/blood supply/*drug effects/metabolism
OTO - NOTNLM
OT  - Apoptosis
OT  - Cyclosporine A
OT  - Germ cell
OT  - Mitochondrial permeability transition pore
OT  - Testicular T/D
EDAT- 2019/06/30 06:00
MHDA- 2020/02/26 06:00
CRDT- 2019/06/29 06:00
PHST- 2018/11/11 00:00 [received]
PHST- 2019/05/20 00:00 [revised]
PHST- 2019/06/14 00:00 [accepted]
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2020/02/26 06:00 [medline]
PHST- 2019/06/29 06:00 [entrez]
AID - S0014-4800(18)30536-7 [pii]
AID - 10.1016/j.yexmp.2019.104271 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2019 Oct;110:104271. doi: 10.1016/j.yexmp.2019.104271. Epub 2019 
      Jun 25.