PMID- 31251959
OWN - NLM
STAT- MEDLINE
DCOM- 20200203
LR  - 20200203
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 708
DP  - 2019 Aug 24
TI  - Directed differentiation of human adipose tissue-derived stem cells to 
      dopaminergic neurons in low-serum and serum-free conditions.
PG  - 134353
LID - S0304-3940(19)30456-2 [pii]
LID - 10.1016/j.neulet.2019.134353 [doi]
AB  - Directing the fate of mesenchymal stem cells (MSCs) to dopaminergic neurons has 
      great importance in both biomedical studies and cell therapy of Parkinson's 
      disease. We recently generated dopamine-secreting cells from human adipose 
      tissue-derived stem cells (hADSCs) by exposing the cells to a growth factor 
      cocktail composed of SHH, bFGF, FGF8 and BDNF in low-serum condition. In the 
      current study, we induced the cells by the same dopaminergic inducing cocktail in 
      serum-free B27-supplemented Neurobasal medium. ADSCs differentiated in both 
      conditions expressed several neuronal and dopaminergic markers. However, there 
      were higher gene expression levels under the serum-free condition. Higher levels 
      of TUJ1 and TH proteins were also detected in the cells exposed to the 
      dopaminergic-inducing cocktail under serum-free Neurobasal condition. TH protein 
      was expressed in about 28% and 60% of the cells differentiated in the low-serum 
      and serum-free Neurobasal media, respectively. Moreover, the cells exposed to the 
      dopaminergic-inducing cocktail in the serum-free Neurobasal condition released a 
      more significant amount of dopamine in response to KCl-induced depolarization. 
      Altogether, these findings show a greater efficiency of the serum-free Neurobasal 
      condition for growth factor-directed differentiation of hADSCs to functional 
      dopamine-secreting cells which may be valuable for transplantation therapy of 
      Parkinson's disease in future.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Faghih, Hossein
AU  - Faghih H
AD  - Department of Stem Cells and Regenerative medicine, Institute for Medical 
      Biotechnology, National Institute of Genetic Engineering and Biotechnology 
      (NIGEB), Tehran, Iran.
FAU - Javeri, Arash
AU  - Javeri A
AD  - Department of Stem Cells and Regenerative medicine, Institute for Medical 
      Biotechnology, National Institute of Genetic Engineering and Biotechnology 
      (NIGEB), Tehran, Iran. Electronic address: arashj@nigeb.ac.ir.
FAU - Amini, Hossein
AU  - Amini H
AD  - Department of Pharmacology, Neuroscience Research Center, Golestan University of 
      Medical Sciences, Gorgan, Iran.
FAU - Taha, Masoumeh Fakhr
AU  - Taha MF
AD  - Department of Stem Cells and Regenerative medicine, Institute for Medical 
      Biotechnology, National Institute of Genetic Engineering and Biotechnology 
      (NIGEB), Tehran, Iran. Electronic address: mftaha@nigeb.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190625
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - Dopaminergic Neurons/*cytology
MH  - Female
MH  - Humans
MH  - Middle Aged
MH  - Stem Cells/*cytology
OTO - NOTNLM
OT  - ADSC
OT  - Differentiation
OT  - Dopaminergic neuron
OT  - Low-serum
OT  - Serum-free
EDAT- 2019/06/30 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/06/29 06:00
PHST- 2019/04/15 00:00 [received]
PHST- 2019/06/22 00:00 [revised]
PHST- 2019/06/24 00:00 [accepted]
PHST- 2019/06/30 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/06/29 06:00 [entrez]
AID - S0304-3940(19)30456-2 [pii]
AID - 10.1016/j.neulet.2019.134353 [doi]
PST - ppublish
SO  - Neurosci Lett. 2019 Aug 24;708:134353. doi: 10.1016/j.neulet.2019.134353. Epub 
      2019 Jun 25.