PMID- 31256666
OWN - NLM
STAT- MEDLINE
DCOM- 20190708
LR  - 20190709
IS  - 2049-4408 (Electronic)
IS  - 2049-4394 (Linking)
VI  - 101-B
IP  - 7
DP  - 2019 Jul
TI  - Multiple intra-articular injections of allogeneic bone marrow-derived stem cells 
      potentially improve knee lesions resulting from surgically induced 
      osteoarthritis: an animal study.
PG  - 824-831
LID - 10.1302/0301-620X.101B7.BJJ-2018-1532.R1 [doi]
AB  - AIM: Mesenchymal stem cells (MSCs) have several properties that may support their 
      use as an early treatment option for osteoarthritis (OA). This study investigated 
      the role of multiple injections of allogeneic bone marrow-derived stem cells 
      (BMSCs) to alleviate the progression of osteoarthritic changes in the various 
      structures of the mature rabbit knee in an anterior cruciate ligament 
      (ACL)-deficient OA model. MATERIALS AND METHODS: Two months after bilateral 
      section of the ACL of Japanese white rabbits aged nine months or more, either 
      phosphate buffered saline (PBS) or 1 x 10(6) MSCs were injected into the knee 
      joint in single or three consecutive doses. After two months, the articular 
      cartilage and meniscus were assessed macroscopically, histologically, and 
      immunohistochemically using collagen I and II. RESULTS: Within the PBS injection 
      (control group), typical progressive degenerative changes were revealed in the 
      various knee structures. In the single MSC injection (single group), 
      osteoarthritic changes were attenuated, but still appeared, especially in the 
      medial compartments involving fibrillation of the articular cartilage, osteophyte 
      formation in the medial plateau, and longitudinal tear of the meniscus. In the 
      multiple-injections group, the smoothness and texture of the articular cartilage 
      and meniscus were improved. Histologically, absence or reduction in matrix 
      staining and cellularity were noticeable in the control and single-injection 
      groups, respectively, in contrast to the multiple-injections group, which showed 
      good intensity of matrix staining and chondrocyte distribution in the various 
      cartilage zones. Osteoarthritis Research Society International (OARSI) scoring 
      showed significantly better results in the multiple-injections group than in the 
      other groups. Immunohistochemically, collagen I existed superficially in the 
      medial femoral condyle in the single group, while collagen II was more evident in 
      the multiple-injections group than the single-injection group. CONCLUSION: A 
      single injection of MSCs was not enough to restore the condition of 
      osteoarthritic joints. This is in contrast to multiple injections of MSCs, which 
      had the ability to replace lost cells, as well as reducing inflammation. Cite 
      this article: Bone Joint J 2019;101-B:824-831.
FAU - Mahmoud, E E
AU  - Mahmoud EE
AD  - Department of Surgery, Faculty of Veterinary Medicine, South Valley University, 
      Qena, Egypt.
FAU - Adachi, N
AU  - Adachi N
AD  - Department of Orthopaedic Surgery, Integrated Health Sciences, Institute of 
      Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Mawas, A S
AU  - Mawas AS
AD  - Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, 
      South Valley University, Qena, Egypt.
FAU - Deie, M
AU  - Deie M
AD  - Department of Orthopaedic Surgery, Aichi Medical University, Aichi, Japan.
FAU - Ochi, M
AU  - Ochi M
AD  - Hiroshima University, Hiroshima, Japan.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Bone Joint J
JT  - The bone & joint journal
JID - 101599229
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/surgery
MH  - Injections, Intra-Articular
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Osteoarthritis, Knee/etiology/pathology/*therapy
MH  - Rabbits
MH  - Transplantation, Homologous
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Allogeneic BMSCs
OT  - Knee lesions
OT  - OA treatment
OT  - Rabbit model
EDAT- 2019/07/02 06:00
MHDA- 2019/07/10 06:00
CRDT- 2019/07/02 06:00
PHST- 2019/07/02 06:00 [entrez]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2019/07/10 06:00 [medline]
AID - 10.1302/0301-620X.101B7.BJJ-2018-1532.R1 [doi]
PST - ppublish
SO  - Bone Joint J. 2019 Jul;101-B(7):824-831. doi: 
      10.1302/0301-620X.101B7.BJJ-2018-1532.R1.