PMID- 31257089
OWN - NLM
STAT- MEDLINE
DCOM- 20200224
LR  - 20200224
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 215
IP  - 9
DP  - 2019 Sep
TI  - Circ-EIF4G3 promotes the development of gastric cancer by sponging miR-335.
PG  - 152507
LID - S0344-0338(19)30650-8 [pii]
LID - 10.1016/j.prp.2019.152507 [doi]
AB  - BACKGROUND: Circular RNAs (circRNAs), a new group of endogenous non-coding RNAs, 
      plays a crucial role in various types of carcinomas. However, there is still 
      limited information on the involvement of circular RNAs in the setting of gastric 
      cancer (GC). In the present study, we aimed to investigate circ-EIF4G3 status in 
      clinical GC patient samples and explored the malignant biological behaviors. 
      MATERIALS: The expression of circ-EIF4G3 was determined by quantitative real time 
      polymerase chain reaction (qRT-PCR). Microarray was performed to detect 
      si-circ-EIF4G3 and unprocessed BGC-823 cells to find a cluster of differently 
      expressed microRNAs (miRNAs) and bioinformatic tools including circinteractome, 
      GO, NHGR1_GWAS, KEGG analyses were used in follow-up analysis. Luciferase 
      reporter, RNA pull down and fluorescence in situ hybridization (FISH) assays were 
      employed to explore the interaction between circ-EIF4G3 and miR-335. 
      SiRNA-mediated knockdown of circ-EIF4G3, proliferation, migration and invasion in 
      vitro were used to evaluate the function of circ-EIF4G3. RESULTS: An increase 
      level in the circ-EIF4G3 expression was associated with higher TNM stage and 
      lymphatic metastasis. In vitro assays of the GC cell lines AGS and BGC-823 
      demonstrated that knockdown of circ-EIF4G3 inhibited cell proliferation, invasion 
      and migration significantly. In addition, circ-EIF4G3 was identified as a sponge 
      of miR-335, further promoting the proliferation, invasion and migration of GC 
      cells. CONCLUSION: Our study demonstrates that circ-EIF4G3 promotes the 
      proliferation, invasion and migration of gastric cancer via sponging miR-335.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier GmbH.. All rights reserved.
FAU - Wang, Qian
AU  - Wang Q
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Wang, Tiegang
AU  - Wang T
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China; Department of General Surgery, Nanjing Red Cross 
      Hospital, Nanjing, China.
FAU - Hu, Yun
AU  - Hu Y
AD  - Department of General Surgery, Jiangsu Cancer Hospital & Jiangsu Institute of 
      Cancer Research & The Affiliated Cancer Hospital of Nanjing Medical University, 
      Nanjing, China.
FAU - Jiang, Wei
AU  - Jiang W
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Lu, Chen
AU  - Lu C
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Zheng, Wubin
AU  - Zheng W
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China.
FAU - Zhang, Wenling
AU  - Zhang W
AD  - Department of Gastroenterology, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China. Electronic address: 1510626230@qq.com.
FAU - Chen, Ziyi
AU  - Chen Z
AD  - Department of General Surgery, Zhongda Hospital, Medical School, Southeast 
      University, Nanjing, Jiangsu, China. Electronic address: dr_czy@126.com.
FAU - Cao, Hongyong
AU  - Cao H
AD  - Department of General Surgery, Nanjing First Hospital, Nanjing Medical 
      University, Nanjing, China. Electronic address: caohongy6167@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190620
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Eukaryotic Initiation Factor-4G)
RN  - 0 (MIRN335 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
EIN - Pathol Res Pract. 2021 Jan;217:153163. PMID: 33272720
MH  - Adult
MH  - Aged
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Eukaryotic Initiation Factor-4G/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - RNA, Circular/*genetics
MH  - Stomach Neoplasms/genetics/*pathology
OTO - NOTNLM
OT  - Gastric carcinoma
OT  - Invasion
OT  - Migration
OT  - Sponge
EDAT- 2019/07/02 06:00
MHDA- 2020/02/25 06:00
CRDT- 2019/07/02 06:00
PHST- 2019/04/09 00:00 [received]
PHST- 2019/05/27 00:00 [revised]
PHST- 2019/06/20 00:00 [accepted]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2020/02/25 06:00 [medline]
PHST- 2019/07/02 06:00 [entrez]
AID - S0344-0338(19)30650-8 [pii]
AID - 10.1016/j.prp.2019.152507 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2019 Sep;215(9):152507. doi: 10.1016/j.prp.2019.152507. Epub 
      2019 Jun 20.