PMID- 31257517
OWN - NLM
STAT- MEDLINE
DCOM- 20191223
LR  - 20210311
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 20
IP  - 2
DP  - 2019 Aug
TI  - Long non‑coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme.
PG  - 1873-1882
LID - 10.3892/mmr.2019.10428 [doi]
AB  - Glioblastoma multiforme (GBM) is the most common malignant tumor with high 
      morbidity and mortality. This study investigated the role of long non‑coding RNAs 
      (lncRNAs) in glioblastomagenesis progression. Using the GSE2223 and GSE59612 
      datasets, and RNA sequencing data of GBM from The Cancer Genome Atlas, 
      differentially expressed (DE) genes including DE messenger RNAs (DEmRNAs) and 
      DElncRNAs between GBM and normal controls were identified. Based on the competing 
      endogenous RNA hypothesis, DElncRNA‑micro RNA (miRNA)‑DEmRNA interactions were 
      obtained by target gene prediction. Gene Ontology (GO) and Kyoto Encyclopedia of 
      Gene and Genomes pathway analysis of DEmRNAs in the DElncRNA‑miRNA‑DEmRNA network 
      was performed. Expression and function analyses of DElncRNAs were performed by 
      reverse transcription‑polymerase chain reaction (RT‑PCR) and an established 
      viability assay, respectively. In total, 712 DE genes were identified. 
      Significant upregulation of lncRNA deleted in lymphocytic leukemia 1 (DLEU1) was 
      revealed in GBM and a number of other types of cancer. DLEU1 interacted with 315 
      miRNAs and 105 DEmRNAs. The DEmRNAs were mainly enriched in 
      tumorigenesis‑associated GO terms (angiogenesis, positive regulation of cell 
      proliferation, positive regulation of fibroblast apoptotic processes and 
      regulation of neutrophil migration) and pathways (Hippo signaling pathway, cancer 
      pathways, and Wnt signaling pathway). Correlation analysis revealed that mRNA TNF 
      receptor associated factor 4 (TRAF4) was associated with DLEU1 expression. RT‑PCR 
      demonstrated that the expression levels of DLEU1 and TRAF4 were increased in GBM 
      tissues. Small interfering RNA demonstrated that silencing DLEU1 downregulated 
      TRAF4. The viability of GBM cells was significantly decreased following RNA 
      interference with DLEU1 and TRAF4 production. The results demonstrate that DLEU1 
      and TRAF4 is highly expressed in GBM tissues and promotes proliferation of GBM 
      cells. It may act as a competing endogenous RNA and influence tumorigenesis of 
      GBM.
FAU - Wang, Jiancun
AU  - Wang J
AD  - Department of Neurosurgery, People's Hospital of Zhangjiajie, Zhangjiajie, Hunan 
      427000, P.R. China.
FAU - Quan, Xingyun
AU  - Quan X
AD  - Department of Neurosurgery, People's Hospital of Zhangjiajie, Zhangjiajie, Hunan 
      427000, P.R. China.
FAU - Peng, Dingting
AU  - Peng D
AD  - Department of Neurosurgery, People's Hospital of Zhangjiajie, Zhangjiajie, Hunan 
      427000, P.R. China.
FAU - Hu, Guancheng
AU  - Hu G
AD  - Department of Neurosurgery, People's Hospital of Zhangjiajie, Zhangjiajie, Hunan 
      427000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190626
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (DLEU1 lncRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
RN  - 0 (TNF Receptor-Associated Factor 4)
RN  - 0 (TRAF4 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Adult
MH  - Carcinogenesis/genetics
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Gene Regulatory Networks/genetics
MH  - Glioblastoma/*genetics/pathology
MH  - Humans
MH  - Male
MH  - MicroRNAs/genetics
MH  - Middle Aged
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Messenger/genetics
MH  - Sequence Analysis, RNA
MH  - TNF Receptor-Associated Factor 4/*genetics
MH  - Tumor Suppressor Proteins/*genetics
EDAT- 2019/07/02 06:00
MHDA- 2019/12/24 06:00
CRDT- 2019/07/02 06:00
PHST- 2018/06/04 00:00 [received]
PHST- 2019/01/10 00:00 [accepted]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2019/12/24 06:00 [medline]
PHST- 2019/07/02 06:00 [entrez]
AID - 10.3892/mmr.2019.10428 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Aug;20(2):1873-1882. doi: 10.3892/mmr.2019.10428. Epub 2019 Jun 
      26.