PMID- 31258650
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 1
DP  - 2019 Jul
TI  - Angiogenesis and bone regeneration by mesenchymal stem cell transplantation with 
      danshen in a rabbit model of avascular necrotic femoral head.
PG  - 163-171
LID - 10.3892/etm.2019.7556 [doi]
AB  - The present study aimed to explore the potential of combined treatment with 
      mesenchymal stem cells (MSCs) and danshen for angiogenesis and bone regeneration 
      in a rabbit model of avascular necrosis of femoral head (ANFH). A rabbit model of 
      ANFH was established using the Shwartzman reaction with methylprednisolone and 
      Escherichia coli endotoxin injection. Magnetic resonance imaging (MRI) and 
      histopathological examination were used to evaluate the rabbit model of ANFH. The 
      rabbits were randomly divided into the danshen group, the MSCs group, the danshen 
      combined with MSCs group and the model group (treated with physiological saline). 
      The expression level of monocyte chemoattractant protein-1 (MCP-1) and stromal 
      cell-derived factor-1 (SDF-1) were determined by reverse transcription polymerase 
      chain reaction (RT-PCR). The expression level of bone morphogenetic protein-2 
      (BMP-2) and vascular endothelial growth factor (VEGF) were detected by 
      immunofluorescence and the mRNA expression of BMP-2 and VEGF were detected by 
      RT-PCR. Typical osteonecrosis occurred in the rabbit model of ANFH, which 
      indicated that the model was successfully established. MCP-1 and SDF-1 were 
      significantly increased in the model group compared with the normal group 
      (P<0.05). Following the administration of MSCs and Salvia miltiorrhiza (danshen), 
      MSCs labeled with 5-bromo-2-deoxyuridine were observed to be gathered in the 
      necrotic area. The increased migration of MSCs to the necrotic area may be due to 
      the upregulated expression of the chemokines MCP-1 and SDF-1. ANFH treated with 
      danshen combined with MSCs may promote revascularization by increasing the 
      expression of VEGF and BMP-2 in the femoral head, promoting re-ossification and 
      revascularization. Danshen combined with the transplantation of MSCs may be 
      regarded as a novel therapy for the treatment of ANFH in a clinical setting.
FAU - Wu, Yungang
AU  - Wu Y
AD  - Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Zhang, Chunwu
AU  - Zhang C
AD  - Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Wu, Jianjing
AU  - Wu J
AD  - Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Han, Yan
AU  - Han Y
AD  - Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
FAU - Wu, Chunlei
AU  - Wu C
AD  - Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190508
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6566092
OTO - NOTNLM
OT  - avascular necrosis of femoral head
OT  - bone morphogenetic protein-2
OT  - danshen
OT  - mesenchymal stem cells
OT  - monocyte chemoattractant protein-1
OT  - stromal cell-derived factor-1
OT  - vascular endothelial growth factor
EDAT- 2019/07/02 06:00
MHDA- 2019/07/02 06:01
PMCR- 2019/05/08
CRDT- 2019/07/02 06:00
PHST- 2017/06/29 00:00 [received]
PHST- 2017/11/03 00:00 [accepted]
PHST- 2019/07/02 06:00 [entrez]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2019/07/02 06:01 [medline]
PHST- 2019/05/08 00:00 [pmc-release]
AID - ETM-0-0-7556 [pii]
AID - 10.3892/etm.2019.7556 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Jul;18(1):163-171. doi: 10.3892/etm.2019.7556. Epub 2019 May 
      8.