PMID- 31258651
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 1
DP  - 2019 Jul
TI  - Optimal ratio of 18alpha- and 18beta-glycyrrhizic acid for preventing alcoholic 
      hepatitis in rats.
PG  - 172-178
LID - 10.3892/etm.2019.7572 [doi]
AB  - The glycyrrhizic acid (GA) epimers 18alpha- and 18beta-GA exert anti-inflammatory and 
      hepatoprotective activities, which may help to protect against alcoholic liver 
      disease, particularly alcoholic hepatitis (AH). The aim of the present study was 
      to investigate the optimal ratio of 18alpha- and 18beta-GA for preventing AH in rats. 
      Different groups of rats were administered seven different ratios of 18alpha- and 
      18beta-GA (10:0, 8:2, 6:4, 5:5, 4:6, 2:8 and 0:10; 10.83 mg/kg), vehicle control, or 
      silymarin (22.75 mg/kg) as a positive control, followed by administration of 40% 
      alcohol (10 ml/kg) once a day for four weeks. Subsequently, livers were isolated 
      and routinely processed for histological examination. The serum levels of 23 
      cytokines and chemokines associated with AH were examined with a Bio-Plex 200 
      Luminex assay. It was revealed that all ratios of 18alpha- and 18beta-GA prevented 
      alcohol-induced liver injury, as evidenced by a lesser degree of 
      histopathological changes in the liver as compared with those in the model group. 
      Furthermore, the levels of 15 cytokines/chemokines were significantly altered 
      after alcohol administration, which was significantly inhibited by, pre-treatment 
      with different proportions of 18alpha- and 18beta-GA, particularly at a ratio of 4:6, 
      for most cytokines/chemokines associated with AH, including tumor necrosis factor 
      (TNF)-alpha, interleukin (IL)-1beta, IL-2, IL-4, IL-5, IL-7, IL-6, monocyte chemotactic 
      protein 1 (MCP-1), macrophage inflammatory protein (MIP)-1alpha, MIP-3alpha, macrophage- 
      and granulocyte macrophage colony-stimulating factor, chemokine (C-X-C motif) 
      ligand 1(GRO/KC), vascular endothelial growth factor and C-C motif chemokine 
      ligand 5 (RANTES). Taken together, based on these results the optimal ratio of 
      18alpha- and 18beta-GA to prevent AH in model rats was considered to be 4:6.
FAU - Huo, Xiaowei
AU  - Huo X
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
FAU - Sun, Xiaoke
AU  - Sun X
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
FAU - Cao, Zepeng
AU  - Cao Z
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
FAU - Qiao, Jingzhe
AU  - Qiao J
AD  - Oncology Department, Hebei Yiling Hospital, Shijiazhuang, Hebei 050091, P.R. 
      China.
FAU - Yang, Sa
AU  - Yang S
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
FAU - Meng, Xiangbo
AU  - Meng X
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
FAU - Zhao, Yanyan
AU  - Zhao Y
AD  - College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality 
      Control of Hebei Province, Hebei University, Baoding, Hebei 071002, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190510
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6566121
OTO - NOTNLM
OT  - 18alpha- and 18beta-glycyrrhizic acid
OT  - alcoholic hepatitis
OT  - chemokines
OT  - cytokines
OT  - optimal ratio
EDAT- 2019/07/02 06:00
MHDA- 2019/07/02 06:01
PMCR- 2019/05/10
CRDT- 2019/07/02 06:00
PHST- 2018/05/08 00:00 [received]
PHST- 2018/11/07 00:00 [accepted]
PHST- 2019/07/02 06:00 [entrez]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2019/07/02 06:01 [medline]
PHST- 2019/05/10 00:00 [pmc-release]
AID - ETM-0-0-7572 [pii]
AID - 10.3892/etm.2019.7572 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Jul;18(1):172-178. doi: 10.3892/etm.2019.7572. Epub 2019 May 
      10.