PMID- 3125866
OWN - NLM
STAT- MEDLINE
DCOM- 19880414
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 71
IP  - 3
DP  - 1988 Mar
TI  - The beta subunit of the interleukin-2 receptor mediates interleukin-2 induction 
      of anti-CD3 redirected cytotoxic capability in large granular lymphocytes.
PG  - 825-8
AB  - The interleukin 2 (IL 2) receptor was studied in three cases of large granular 
      lymphocyte (LGL) lymphocytosis. All cases were nonreactive with anti-Tac 
      monoclonal antibody (MoAb; recognizing the p55 alpha subunit of the IL 2 
      receptor). Sodium dodecyl sulfate (SDS)/polyacrylamide gel electrophoretic 
      analysis (PAGE) of cells to which radio-labeled rIL 2 had been chemically 
      crosslinked revealed uniform expression of the p70/75 beta subunit of the IL 2 
      receptor in the absence of the alpha subunit. Stimulation of this receptor with 2 
      nmol/L rIL 2 for five days led to acquisition of anti-CD3 redirected 
      cytotoxicity. This was accompanied by a fivefold to tenfold elevation in the 
      activity of intracellular N-alpha-benzyloxycarbonyl-L-lysine thiobenzyl esterase, 
      an LGL granule marker enzyme. These effects of IL 2 did not require induction of 
      the Tac peptide.
FAU - Colamonici, O R
AU  - Colamonici OR
AD  - Laboratory of Pathology, National Cancer Institute--Naval Medical Oncology 
      Branch, Bethesda, MD 20892.
FAU - Quinones, R
AU  - Quinones R
FAU - Rosolen, A
AU  - Rosolen A
FAU - Trepel, J B
AU  - Trepel JB
FAU - Sausville, E
AU  - Sausville E
FAU - Phares, J C
AU  - Phares JC
FAU - Gress, R
AU  - Gress R
FAU - Poplack, D
AU  - Poplack D
FAU - Weber, J
AU  - Weber J
FAU - Schechter, G P
AU  - Schechter GP
AU  - et al.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, Surface)
RN  - 0 (Interleukin-2)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, Interleukin-2)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Antigens, Surface/*immunology
MH  - *Cytotoxicity, Immunologic
MH  - Humans
MH  - Interleukin-2/*pharmacology
MH  - Lymphocytes/*immunology/metabolism
MH  - Receptors, Immunologic/metabolism/*physiology
MH  - Receptors, Interleukin-2
MH  - Recombinant Proteins
EDAT- 1988/03/01 00:00
MHDA- 1988/03/01 00:01
CRDT- 1988/03/01 00:00
PHST- 1988/03/01 00:00 [pubmed]
PHST- 1988/03/01 00:01 [medline]
PHST- 1988/03/01 00:00 [entrez]
AID - S0006-4971(20)78167-X [pii]
PST - ppublish
SO  - Blood. 1988 Mar;71(3):825-8.