PMID- 31260679
OWN - NLM
STAT- MEDLINE
DCOM- 20200824
LR  - 20200824
IS  - 1873-507X (Electronic)
IS  - 0031-9384 (Linking)
VI  - 209
DP  - 2019 Oct 1
TI  - Role of orexin receptors within the dentate gyrus of the hippocampus in 
      antinociception induced by chemical stimulation of the lateral hypothalamus in 
      the tail-flick test as a model of acute pain in rats.
PG  - 112595
LID - S0031-9384(19)30378-6 [pii]
LID - 10.1016/j.physbeh.2019.112595 [doi]
AB  - The dentate gyrus (DG) as a part of the hippocampal formation is considered one 
      of the brain areas involved in pain perception. The orexin neurons in the lateral 
      hypothalamus (LH) also show connections to regions engaged in the circuitry of 
      pain modulation and many studies have shown their potential to alter pain 
      transmission through the nervous system. However, the role of orexin receptors 
      (OXRs) of the DG in mediation of antinociceptive responses induced by the LH 
      stimulation in rats has not yet been investigated. Hence, the present study was 
      an attempt to examine the role of OXRs located within the DG in the pain 
      modulation induced by chemical stimulation of the LH. Adult male Wistar rats 
      weighing 220-250 g were unilaterally implanted with two separate cannulae into 
      the LH and DG. Intra-DG administration of the orexin-1 receptor (OX1R) 
      antagonist, SB334867 or the orexin-2 receptor (OX2R) antagonist, TCS OX2 29 was 
      performed just 5 min before intra-LH carbachol microinjection. Antinociceptive 
      effects were measured using the tail-flick apparatus. The results showed that 
      OX1R and OX2R antagonists dose-dependently decreased the antinociceptive effect 
      of carbachol. In addition, the effective dose of SB334867 was lesser than that of 
      TCS OX2 29, which indicates the more prominent role of OX1R of the DG in 
      carbachol-induced antinociception. None of the antagonists had any significant 
      effect when administrated without intra-LH carbachol microinjection. It seems to 
      be a neural circuit includes the LH and DG, which plays a major role in the 
      neural basis of pain modulation.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Brojeni, Masoud Shareghi
AU  - Brojeni MS
AD  - Department of Physiology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran.
FAU - Rashvand, Mina
AU  - Rashvand M
AD  - Department of Physiology, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran.
FAU - Haghparast, Abbas
AU  - Haghparast A
AD  - Neuroscience Research Center, School of Medicine, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. Electronic address: Haghparast@sbmu.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190629
PL  - United States
TA  - Physiol Behav
JT  - Physiology & behavior
JID - 0151504
RN  - 0 (1-(2-methylbenzoxazol-6-yl)-3-(1,5)naphthyridin-4-yl urea)
RN  - 0 
      (1-(3,4-dihydro-6,7-dimethoxy-2(1H)-isoquinolinyl)-3,3-dimethyl-2-((4-pyridinylmethyl)amino)-1-butanone)
RN  - 0 (Analgesics)
RN  - 0 (Benzoxazoles)
RN  - 0 (Hcrtr1 protein, rat)
RN  - 0 (Hcrtr2 protein, rat)
RN  - 0 (Isoquinolines)
RN  - 0 (Naphthyridines)
RN  - 0 (Orexin Receptor Antagonists)
RN  - 0 (Orexin Receptors)
RN  - 0 (Pyridines)
RN  - 8W8T17847W (Urea)
RN  - 8Y164V895Y (Carbachol)
SB  - IM
MH  - Acute Pain/*psychology
MH  - Analgesics/pharmacology
MH  - Animals
MH  - Benzoxazoles/pharmacology
MH  - Carbachol/pharmacology
MH  - Dentate Gyrus/*physiopathology
MH  - Dose-Response Relationship, Drug
MH  - Isoquinolines
MH  - Male
MH  - Motor Activity/drug effects
MH  - Naphthyridines/pharmacology
MH  - Nociception/*drug effects
MH  - Orexin Receptor Antagonists/pharmacology
MH  - *Orexin Receptors/drug effects
MH  - Pain Measurement/*drug effects
MH  - Pyridines
MH  - Rats
MH  - Rats, Wistar
MH  - Stimulation, Chemical
MH  - Urea/analogs & derivatives/pharmacology
OTO - NOTNLM
OT  - Dentate gyrus
OT  - Lateral hypothalamus
OT  - Orexin
OT  - Pain
OT  - Rat
OT  - Tail-flick test
EDAT- 2019/07/02 06:00
MHDA- 2020/08/25 06:00
CRDT- 2019/07/02 06:00
PHST- 2019/04/10 00:00 [received]
PHST- 2019/06/28 00:00 [revised]
PHST- 2019/06/28 00:00 [accepted]
PHST- 2019/07/02 06:00 [pubmed]
PHST- 2020/08/25 06:00 [medline]
PHST- 2019/07/02 06:00 [entrez]
AID - S0031-9384(19)30378-6 [pii]
AID - 10.1016/j.physbeh.2019.112595 [doi]
PST - ppublish
SO  - Physiol Behav. 2019 Oct 1;209:112595. doi: 10.1016/j.physbeh.2019.112595. Epub 
      2019 Jun 29.