PMID- 31260839 OWN - NLM STAT- MEDLINE DCOM- 20191122 LR - 20191122 IS - 1532-2661 (Electronic) IS - 0034-5288 (Linking) VI - 125 DP - 2019 Aug TI - Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus. PG - 195-204 LID - S0034-5288(19)30010-4 [pii] LID - 10.1016/j.rvsc.2019.06.011 [doi] AB - Peste des petits ruminants virus (PPRV) poses a great threat to livestock husbandry, especially goat farming due to its high mortality and morbidity. Dendritic cells (DCs), as the principal stimulators of naive Th cells were widely used in antigen processing and presenting. In the previous study, we tested the effects of PPRV on murine bone marrow derived dendritic cells (BMDCs) including surface markers and cytokines. While the aim of this study is to detect the proteomic profile of BMDCs stimulated with PPRV towards key proteins involved in. Following PPRV stimulation, 110 differentially expressed proteins (DEPs) were identified through iTRAQ labelling with LC-MS/MS approach, of which 94 DEPs were up-regulated and 16 DEPs were down-regulated, respectively. Among them 15 out of 110 DGPs were related to innate immune system, three were involved in cell apoptosis, RPS15a and Smox were related to translation of viral mRNA. Additionally, western blot analysis showed identical results to iTRAQ analysis. There will be profound significance for understanding antigen-presenting of BMDCs after stimulation with PPRV. CI - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Li, Lingxia AU - Li L AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. FAU - Wu, Jinyan AU - Wu J AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: wujinyan@caas.cn. FAU - Cao, Xiao''an AU - Cao X AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: caoxiaoan@caas.cn. FAU - Zhou, Jianhua AU - Zhou J AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: Zhoujianhua03@caas.cn. FAU - Yin, Shuanghui AU - Yin S AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: yinshuanghui@caas.cn. FAU - Yang, Shunli AU - Yang S AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: yangshunli@caas.cn. FAU - Feng, Qian AU - Feng Q AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. FAU - Du, Ping AU - Du P AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. FAU - Liu, Yongjie AU - Liu Y AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: liuyongjie02@caas.cn. FAU - Shang, Youjun AU - Shang Y AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: shangyoujun@caas.cn. FAU - Liu, Yongsheng AU - Liu Y AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: liuyongsheng@caas.cn. FAU - Liu, Xiangtao AU - Liu X AD - State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of the Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China. Electronic address: liuxiangtao@caas.cn. LA - eng PT - Journal Article DEP - 20190620 PL - England TA - Res Vet Sci JT - Research in veterinary science JID - 0401300 RN - 0 (RNA, Messenger) SB - IM MH - Animals MH - Blotting, Western MH - Bone Marrow Cells/*physiology MH - Chromatography, Liquid MH - Dendritic Cells/*physiology MH - Female MH - Mice MH - Mice, Inbred BALB C MH - *Peste-des-petits-ruminants virus MH - *Proteomics MH - RNA, Messenger MH - Tandem Mass Spectrometry OTO - NOTNLM OT - Bone marrow derived dendritic cells OT - Peste des petits ruminants virus OT - Proteomics OT - iTRAQ EDAT- 2019/07/02 06:00 MHDA- 2019/11/23 06:00 CRDT- 2019/07/02 06:00 PHST- 2019/01/15 00:00 [received] PHST- 2019/06/11 00:00 [revised] PHST- 2019/06/18 00:00 [accepted] PHST- 2019/07/02 06:00 [pubmed] PHST- 2019/11/23 06:00 [medline] PHST- 2019/07/02 06:00 [entrez] AID - S0034-5288(19)30010-4 [pii] AID - 10.1016/j.rvsc.2019.06.011 [doi] PST - ppublish SO - Res Vet Sci. 2019 Aug;125:195-204. doi: 10.1016/j.rvsc.2019.06.011. Epub 2019 Jun 20.