PMID- 31261673
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231013
IS  - 1999-4923 (Print)
IS  - 1999-4923 (Electronic)
IS  - 1999-4923 (Linking)
VI  - 11
IP  - 7
DP  - 2019 Jun 28
TI  - Latest Advances in the Development of Eukaryotic Vaults as Targeted Drug Delivery 
      Systems.
LID - 10.3390/pharmaceutics11070300 [doi]
LID - 300
AB  - The use of smart drug delivery systems (DDSs) is one of the most promising 
      approaches to overcome some of the drawbacks of drug-based therapies, such as 
      improper biodistribution and lack of specific targeting. Some of the most 
      attractive candidates as DDSs are naturally occurring, self-assembling protein 
      nanoparticles, such as viruses, virus-like particles, ferritin cages, bacterial 
      microcompartments, or eukaryotic vaults. Vaults are large ribonucleoprotein 
      nanoparticles present in almost all eukaryotic cells. Expression in different 
      cell factories of recombinant versions of the "major vault protein" (MVP) results 
      in the production of recombinant vaults indistinguishable from native 
      counterparts. Such recombinant vaults can encapsulate virtually any cargo 
      protein, and they can be specifically targeted by engineering the C-terminus of 
      MVP monomer. These properties, together with nanometric size, a lumen large 
      enough to accommodate cargo molecules, biodegradability, biocompatibility and no 
      immunogenicity, has raised the interest in vaults as smart DDSs. In this work we 
      provide an overview of eukaryotic vaults as a new, self-assembling protein-based 
      DDS, focusing in the latest advances in the production and purification of this 
      platform, its application in nanomedicine, and the current preclinical and 
      clinical assays going on based on this nanovehicle.
FAU - Munoz-Juan, Amanda
AU  - Munoz-Juan A
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain.
FAU - Carreno, Aida
AU  - Carreno A
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain.
FAU - Mendoza, Rosa
AU  - Mendoza R
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain.
AD  - Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 
      28029 Madrid, Spain.
FAU - Corchero, Jose L
AU  - Corchero JL
AUID- ORCID: 0000-0002-6109-144X
AD  - Institut de Biotecnologia i de Biomedicina, Universitat Autonoma de Barcelona, 
      08193 Bellaterra, Spain. jlcorchero@ciber-bbn.es.
AD  - Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 
      28029 Madrid, Spain. jlcorchero@ciber-bbn.es.
AD  - Department of Genetics and Microbiology, Universitat Autonoma de Barcelona, 08193 
      Bellaterra, Spain. jlcorchero@ciber-bbn.es.
LA  - eng
GR  - PI17/00553/Ministerio de Economia y Competitividad/
GR  - Development of an antitumor protein delivery system into ovarian cancer cells 
      using the subcellular vault/Fundacion Cientifica Asociacion Espanola Contra el 
      Cancer/
GR  - PANCREATOR/Centro de Investigacion Biomedica en Red en Bioingenieria, 
      Biomateriales y Nanomedicina/
PT  - Journal Article
PT  - Review
DEP - 20190628
PL  - Switzerland
TA  - Pharmaceutics
JT  - Pharmaceutics
JID - 101534003
PMC - PMC6680493
OTO - NOTNLM
OT  - drug delivery systems
OT  - eukaryotic vaults
OT  - nanocage
OT  - nanoparticle
OT  - protein self-assembly
COIS- The authors declare no conflicts of interest.
EDAT- 2019/07/03 06:00
MHDA- 2019/07/03 06:01
PMCR- 2019/07/01
CRDT- 2019/07/03 06:00
PHST- 2019/05/28 00:00 [received]
PHST- 2019/06/21 00:00 [revised]
PHST- 2019/06/26 00:00 [accepted]
PHST- 2019/07/03 06:00 [entrez]
PHST- 2019/07/03 06:00 [pubmed]
PHST- 2019/07/03 06:01 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - pharmaceutics11070300 [pii]
AID - pharmaceutics-11-00300 [pii]
AID - 10.3390/pharmaceutics11070300 [doi]
PST - epublish
SO  - Pharmaceutics. 2019 Jun 28;11(7):300. doi: 10.3390/pharmaceutics11070300.