PMID- 31263109
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20210110
IS  - 2041-4889 (Electronic)
VI  - 10
IP  - 7
DP  - 2019 Jul 1
TI  - The P-selectin and PSGL-1 axis accelerates atherosclerosis via activation of 
      dendritic cells by the TLR4 signaling pathway.
PG  - 507
LID - 10.1038/s41419-019-1736-5 [doi]
LID - 507
AB  - P-selectin and dendritic cells (DCs) are associated with atherosclerosis. 
      However, their interactions in this setting are undefined. Herein, we 
      investigated the role of P-selectin and its receptor P-selectin glycoprotein 
      ligand (PSGL)-1 on atherosclerosis via activation of DCs. In the current study, a 
      total of 34 patients with ST elevation myocardial infarction (STEMI) and 34 
      healthy control subjects were enrolled. Serum concentration of P-selectin was 
      higher and the myeloid DC/plasmacytoid DC (mDC/pDC) ratio was lower in STEMI 
      patients than in normal individuals. Interestingly, in STEMI patients, P-selectin 
      was decreased and the mDC/pDC ratio was increased at 5-7 days after successful 
      percutaneous coronary intervention, as compared with values on admission. Serum 
      P-selectin was inversely correlated with the mDC/pDC ratio. Moreover, 
      ApoE(-/-)P(-/-) and ApoE(-/-)PSGL-1(-/-) mice developed small atherosclerotic 
      plaques after feeding of a western diet for 12 weeks and DC infiltration was 
      significantly reduced. P-selectin stimulation markedly induced phenotypic 
      maturation, enhanced secretion of inflammatory cytokines, communication with T 
      cells, and the adhesion and migration of DCs. In vivo, DC maturation was 
      significantly attenuated in P-selectin and PSGL1 knockout mice under 
      hypercholesterolemic and inflammatory conditions. These effects were associated 
      with the activation of myeloid differentiation primary response 88 
      (MYD88)-dependent and MyD88-independent Toll-like receptor 4 (TLR4) signaling 
      pathways. Taken together, binding of P-selectin to PSGL-1 on DCs contributes to 
      atherosclerosis progression via DC activation via the TLR4 signaling pathway.
FAU - Ye, Zhishuai
AU  - Ye Z
AUID- ORCID: 0000-0002-8446-1749
AD  - Cardiac Center/Division of Cardiovascular Diseases, Beijing Friendship Hospital, 
      Capital Medical University, 100050, Beijing, China.
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China.
FAU - Zhong, Lei
AU  - Zhong L
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China.
FAU - Zhu, Shengnan
AU  - Zhu S
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China.
FAU - Wang, Yinuo
AU  - Wang Y
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China.
FAU - Zheng, Jie
AU  - Zheng J
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China.
FAU - Wang, Shujing
AU  - Wang S
AD  - Department of Biochemistry and Molecular Biology, Dalian Medical University, 
      116044, Dalian, China.
FAU - Zhang, Jianing
AU  - Zhang J
AD  - College of Life Sciences and Pharmacy, Dalian University of Technology, 116027, 
      Dalian, China.
FAU - Huang, Rongchong
AU  - Huang R
AUID- ORCID: 0000-0002-2607-2112
AD  - Cardiac Center/Division of Cardiovascular Diseases, Beijing Friendship Hospital, 
      Capital Medical University, 100050, Beijing, China. rchuang@ccmu.edu.cn.
AD  - Department of Cardiology, The First Affiliated Hospital of Dalian Medical 
      University, 116011, Dalian, China. rchuang@ccmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190701
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (Apolipoproteins E)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (P-Selectin)
RN  - 0 (P-selectin ligand protein)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Apolipoproteins E/genetics/metabolism
MH  - Atherosclerosis/genetics/*metabolism
MH  - B7-2 Antigen/metabolism
MH  - Dendritic Cells/*metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Mice, Knockout
MH  - Middle Aged
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - P-Selectin/genetics/*metabolism
MH  - Signal Transduction/genetics/physiology
MH  - Toll-Like Receptor 4/genetics/*metabolism
PMC - PMC6602970
COIS- The authors declare that they have no conflict of interest.
EDAT- 2019/07/03 06:00
MHDA- 2020/07/14 06:00
PMCR- 2019/07/01
CRDT- 2019/07/03 06:00
PHST- 2019/01/24 00:00 [received]
PHST- 2019/06/06 00:00 [accepted]
PHST- 2019/06/05 00:00 [revised]
PHST- 2019/07/03 06:00 [entrez]
PHST- 2019/07/03 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2019/07/01 00:00 [pmc-release]
AID - 10.1038/s41419-019-1736-5 [pii]
AID - 1736 [pii]
AID - 10.1038/s41419-019-1736-5 [doi]
PST - epublish
SO  - Cell Death Dis. 2019 Jul 1;10(7):507. doi: 10.1038/s41419-019-1736-5.