PMID- 31263683
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200309
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 9
DP  - 2019
TI  - How Ocular Surface Microbiota Debuts in Type 2 Diabetes Mellitus.
PG  - 202
LID - 10.3389/fcimb.2019.00202 [doi]
LID - 202
AB  - High glucose represents a good environment for bacterial growth on the skin, on 
      the ocular surface (OS) and in the tears of type 2 diabetes mellitus (T2DM) 
      patients, affecting the conjunctival bacterial community. This study aimed to 
      investigate the OS bacterial flora of T2DM patients and healthy subjects using 
      16S rRNA sequencing-based bacterial identification. Among 23 healthy subjects 
      (CON) and 31 T2DM patients, 54 eyes were examined to investigate the OS bacterial 
      community. Factors potentially affecting the microbial growth were controlled. 
      Results showed the OS microbiota presented higher diversity in the T2DM group 
      than in the CON group. Bioinformatic analysis showed a lower abundance of 
      Proteobacteria and a higher abundance of Bacteroidetes at the phyla level as well 
      as a significantly increased abundance of Acinetobacter and Pseudomonas at the 
      genus level in the T2DM group. The difference in OS microbiota at taxonomic level 
      was associated with Ocular Surface Disease Index and course of T2DM. These 
      findings indicate the OS flora in T2DM patients is significantly different from 
      that in healthy subjects, which may be closely associated with OS discomfort and 
      course of T2DM.
FAU - Li, Siqi
AU  - Li S
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Yi, Guoguo
AU  - Yi G
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Peng, Hui
AU  - Peng H
AD  - School of Nursing, Hunan University of Medicine, Hunan, China.
FAU - Li, Zhenhao
AU  - Li Z
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Chen, Shuze
AU  - Chen S
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Zhong, Huimin
AU  - Zhong H
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Chen, Yifan
AU  - Chen Y
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Wang, Zhoucheng
AU  - Wang Z
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Deng, Qixin
AU  - Deng Q
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
FAU - Fu, Min
AU  - Fu M
AD  - Department of Ophthalmology, Zhujiang Hospital of Southern Medical University, 
      Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190617
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Biodiversity
MH  - Conjunctiva/microbiology
MH  - DNA, Bacterial/genetics
MH  - Diabetes Mellitus, Type 2/*complications
MH  - Eye/*microbiology
MH  - Female
MH  - Humans
MH  - Male
MH  - *Microbiota
MH  - Middle Aged
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Surveys and Questionnaires
PMC - PMC6590198
OTO - NOTNLM
OT  - diabetes mellitus
OT  - flora
OT  - infection
OT  - microbiota
OT  - ocular surface
EDAT- 2019/07/03 06:00
MHDA- 2020/02/19 06:00
PMCR- 2019/01/01
CRDT- 2019/07/03 06:00
PHST- 2019/02/24 00:00 [received]
PHST- 2019/05/23 00:00 [accepted]
PHST- 2019/07/03 06:00 [entrez]
PHST- 2019/07/03 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2019.00202 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2019 Jun 17;9:202. doi: 10.3389/fcimb.2019.00202. 
      eCollection 2019.