PMID- 31264160
OWN - NLM
STAT- MEDLINE
DCOM- 20200309
LR  - 20220125
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 46
IP  - 5
DP  - 2019 Oct
TI  - Effects of copper toxicity at different pH and temperatures on the in vitro 
      enzyme activity in blood and liver of fish, Prochilodus lineatus.
PG  - 4933-4942
LID - 10.1007/s11033-019-04944-y [doi]
AB  - Blood and liver from curimbata (Prochilodus lineatus) acclimated at pH 4.5, 7.0 
      and 8.0 and at 20 and 30  degrees C were exposed in vitro to different concentrations of 
      copper (Cu): 98 +/- 0.8 mug Cu L(-1) at pH 4.5 and 16 +/- 0.2 mug Cu L(-1) at pH 8.0 at 
      20  degrees C; 88 +/- 0.8 mug Cu L(-1) at pH 4.5 and 14 +/- 0.5 mug Cu L(-1) at pH 8.0 at 30  degrees C 
      and in 29 mug Cu L(-1) at pH 7.0 at 20 and 30  degrees C for 2 h. The pH affected the 
      levels of glucose and glycogen and in vitro exposure to Cu increased glucose 
      levels and decreased glycogen at 20 and 30  degrees C. Exposures to acid water and Cu in 
      vitro also resulted in an increase in enzyme activity in the blood, hexokinase 
      (HK), pyruvate kinase (PK) and at pH 8.0 decreased the HK activity and increased 
      PK and lactate dehydrogenase (LDH) activities at 20  degrees C. Cu caused an increase in 
      the activities of HK (at pH 4.5) and PK in both pH, 4.5 and 8.0 at 30  degrees C. At 
      20  degrees C, HK (pH 8.0) and glycose-6-phosphate dehydrogenase (G6PDH) (pH 4.5) 
      activities increased and PK (pHs 4.5 and 8.0) and LDH decreased (pH 4.5) in the 
      liver. In vitro, exposure to Cu increased HK and G6PDH at pH 8.0 and PK activity 
      increased in both pH values and LDH increased in pH 7.0. Cu exposure in vitro at 
      30  degrees C, phosphofructokinase (PFK) and PK activities decreased and LDH activity 
      increased in all pH values when compared to fish from the water from its 
      respective pH values. Interactions occurred in blood and liver between the 
      temperature, pH and exposure to copper in vitro. The in vitro tests may 
      constitute an interesting biological model for experimental and applied 
      toxicology, especially in the case of environmental pollution.
FAU - Dos Santos Carvalho, Cleoni
AU  - Dos Santos Carvalho C
AUID- ORCID: 0000-0003-0755-5239
AD  - Universidade Federal de Sao Carlos, Campus Sorocaba, Rodovia Joao Leme dos 
      Santos, km 110, SP-264, Sorocaba, Sao Paulo, CEP 18052-780, Brazil. 
      san-cleo@ufscar.br.
AD  - Programa de Pos-graduacao em Biotecnologia e Monitoramento Ambiental, 
      Universidade Federal de Sao Carlos, Campus Sorocaba, Rodovia Joao Leme dos 
      Santos, km 110, SP-264, Sorocaba, Sao Paulo, CEP 18052-780, Brazil. 
      san-cleo@ufscar.br.
FAU - Fernandes, Marisa Narciso
AU  - Fernandes MN
AD  - Departamento de Ciencias Fisiologicas, Universidade Federal de Sao Carlos, Caixa 
      Postal 676, Rodovia Washington Luis km 235, Sao Carlos, SP, CEP 13565-905, 
      Brazil.
LA  - eng
GR  - 2017/23781-9/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
PT  - Journal Article
DEP - 20190701
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Water Pollutants, Chemical)
RN  - 789U1901C5 (Copper)
RN  - 9005-79-2 (Glycogen)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Carbohydrate Metabolism
MH  - Characiformes/*metabolism
MH  - Copper/metabolism/*toxicity
MH  - Energy Metabolism
MH  - Glucose/metabolism
MH  - Glycogen/metabolism
MH  - Glycolysis/drug effects
MH  - Hexokinase/metabolism
MH  - Hydrogen-Ion Concentration
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Liver/*metabolism
MH  - Oxidation-Reduction
MH  - Phosphofructokinase-1/metabolism
MH  - Pyruvate Kinase/metabolism
MH  - Temperature
MH  - Water Pollutants, Chemical/toxicity
OTO - NOTNLM
OT  - Erythrocytes
OT  - Hexokinase (HK)
OT  - Lactate dehydrogenase (LDH)
OT  - Metal
OT  - Pyruvate kinase (PK)
EDAT- 2019/07/03 06:00
MHDA- 2020/03/10 06:00
CRDT- 2019/07/03 06:00
PHST- 2019/04/16 00:00 [received]
PHST- 2019/06/26 00:00 [accepted]
PHST- 2019/07/03 06:00 [pubmed]
PHST- 2020/03/10 06:00 [medline]
PHST- 2019/07/03 06:00 [entrez]
AID - 10.1007/s11033-019-04944-y [pii]
AID - 10.1007/s11033-019-04944-y [doi]
PST - ppublish
SO  - Mol Biol Rep. 2019 Oct;46(5):4933-4942. doi: 10.1007/s11033-019-04944-y. Epub 
      2019 Jul 1.