PMID- 31265468
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 7
DP  - 2019
TI  - Proteomic analysis of Paracoccidioides brasiliensis complex isolates: Correlation 
      of the levels of differentially expressed proteins with in vivo virulence.
PG  - e0218013
LID - 10.1371/journal.pone.0218013 [doi]
LID - e0218013
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a systemic mycosis commonly found in 
      Latin America that is caused by distinct species of Paracoccidioides genus: 
      Paracoccidioides brasiliensis complex (S1, PS2, PS3 and PS4) and Paracoccidioides 
      lutzii. Its pathobiology has been recently explored by different approaches to 
      clarify the mechanisms of host-pathogen interactions underpinning PCM. The 
      diversity of clinical forms of this disease has been attributed to both host- and 
      fungus-related factors. METHODOLOGY/PRINCIPAL FINDINGS: For better understanding 
      of the molecular underpinnings of host-fungus interactions, we evaluated in vivo 
      virulence of nine Paracoccidioides brasiliensis complex isolates and correlated 
      it to protein expression profiles obtained by two-dimensional gel 
      electrophoresis. Based on the recovery of viable fungi from mouse organs, the 
      isolates were classified as those having low, moderate, or high virulence. Highly 
      virulent isolates overexpressed proteins related to adhesion process and stress 
      response, probably indicating important roles of those fungal proteins in 
      regulating the colonization capacity, survival, and ability to escape host immune 
      system reaction. Moreover, highly virulent isolates exhibited enhanced expression 
      of glycolytic pathway enzymes concomitantly with repressed expression of 
      succinyl-CoA ligase beta chain, a protein related to the tricarboxylic acid 
      cycle. CONCLUSIONS/SIGNIFICANCE: Our findings may point to the mechanisms used by 
      highly virulent P. brasiliensis isolates to withstand host immune reactions and 
      to adapt to transient iron availability as strategies to survive and overcome 
      stress conditions inside the host.
FAU - do Amaral, Cristiane Candida
AU  - do Amaral CC
AD  - Department of Medicine, Discipline of Infectious Diseases, Federal University of 
      Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Fernandes, Geisa Ferreira
AU  - Fernandes GF
AD  - Department of Microbiology, Immunology and Parasitology, Discipline of Cellular 
      Biology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Rodrigues, Anderson Messias
AU  - Rodrigues AM
AD  - Department of Microbiology, Immunology and Parasitology, Discipline of Cellular 
      Biology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
FAU - Burger, Eva
AU  - Burger E
AD  - Department of Microbiology and Immunology, Federal University of Alfenas 
      (UNIFAL), Alfenas, Brazil.
FAU - de Camargo, Zoilo Pires
AU  - de Camargo ZP
AUID- ORCID: 0000-0002-2971-7983
AD  - Department of Microbiology, Immunology and Parasitology, Discipline of Cellular 
      Biology, Federal University of Sao Paulo (UNIFESP), Sao Paulo, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190702
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - Animals
MH  - Fungal Proteins/*immunology
MH  - Gene Expression Regulation, Fungal/*immunology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Paracoccidioides/immunology/isolation & purification/pathogenicity
MH  - Paracoccidioidomycosis/*immunology/pathology
PMC - PMC6605636
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2019/07/03 06:00
MHDA- 2020/02/19 06:00
PMCR- 2019/07/02
CRDT- 2019/07/03 06:00
PHST- 2018/10/18 00:00 [received]
PHST- 2019/05/23 00:00 [accepted]
PHST- 2019/07/03 06:00 [entrez]
PHST- 2019/07/03 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2019/07/02 00:00 [pmc-release]
AID - PONE-D-18-30248 [pii]
AID - 10.1371/journal.pone.0218013 [doi]
PST - epublish
SO  - PLoS One. 2019 Jul 2;14(7):e0218013. doi: 10.1371/journal.pone.0218013. 
      eCollection 2019.