PMID- 31266978
OWN - NLM
STAT- MEDLINE
DCOM- 20201022
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jul 2
TI  - Characterisation of the Selective Reduced Uteroplacental Perfusion (sRUPP) Model 
      of Preeclampsia.
PG  - 9565
LID - 10.1038/s41598-019-45959-6 [doi]
LID - 9565
AB  - Preeclampsia is a complication of pregnancy characterised by gestational 
      hypertension, proteinuria and/or end organ disease. The reduced uteroplacental 
      perfusion (RUPP) model, via partial occlusion of the lower abdominal aorta, 
      mimics insufficient placental perfusion as a primary causal characteristic of 
      preeclampsia. However, a major limitation of the RUPP model is that perfusion is 
      reduced to the entire hindquarters of the rat resulting in hindlimb ischemia. We 
      hypothesised that clipping the uterine and ovarian arteries in the selective 
      (s)RUPP model would provoke signs of preeclampsia while avoiding systemic 
      ischemia. Sham, RUPP or sRUPP procedures were performed in pregnant Sprague 
      Dawley rats on gestational day (GD)14. On GD21 uterine blood flow was 
      significantly reduced in both the RUPP and sRUPP models while aortic flow was 
      reduced only in RUPP. Both models resulted in increased MAP, increased vascular 
      oxidative stress (superoxide generation), increased pro-inflammatory (RANTES) and 
      reduced pro-angiogenic (endoglin) mediators. Vascular compliance and constriction 
      were unaltered in either RUPP or sRUPP groups. In summary, refinements to the 
      RUPP model simultaneously maintain the characteristic phenotype of preeclampsia 
      and avoid peripheral ischemia; providing a useful tool which may be used to 
      increase our knowledge and bring us closer to a solution for women affected by 
      preeclampsia.
FAU - Morton, J S
AU  - Morton JS
AD  - Faculty of Medicine and Dentistry, Dept. of Ob/Gyn, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
AD  - Women and Children's Health Research Institute, Edmonton, AB, T6G 2R3, Canada.
FAU - Levasseur, J
AU  - Levasseur J
AD  - Faculty of Medicine and Dentistry, Dept. of Pediatrics, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
FAU - Ganguly, E
AU  - Ganguly E
AD  - Faculty of Medicine and Dentistry, Dept. of Ob/Gyn, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
AD  - Faculty of Medicine and Dentistry, Dept. of Physiology, University of Alberta, 
      Edmonton, AB, T6G 2H7, Canada.
AD  - Women and Children's Health Research Institute, Edmonton, AB, T6G 2R3, Canada.
FAU - Quon, A
AU  - Quon A
AUID- ORCID: 0000-0003-2334-4618
AD  - Faculty of Medicine and Dentistry, Dept. of Ob/Gyn, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
AD  - Women and Children's Health Research Institute, Edmonton, AB, T6G 2R3, Canada.
FAU - Kirschenman, R
AU  - Kirschenman R
AD  - Faculty of Medicine and Dentistry, Dept. of Ob/Gyn, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
AD  - Women and Children's Health Research Institute, Edmonton, AB, T6G 2R3, Canada.
FAU - Dyck, J R B
AU  - Dyck JRB
AD  - Faculty of Medicine and Dentistry, Dept. of Pediatrics, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada.
FAU - Fraser, G M
AU  - Fraser GM
AD  - Division of Biomedical Sciences, Faculty of Medicine, Memorial University of 
      Newfoundland, St. John's, NL, A1B 3V6, Canada.
FAU - Davidge, S T
AU  - Davidge ST
AD  - Faculty of Medicine and Dentistry, Dept. of Ob/Gyn, University of Alberta, 
      Edmonton, AB, T6G 2S2, Canada. sandra.davidge@ualberta.ca.
AD  - Faculty of Medicine and Dentistry, Dept. of Physiology, University of Alberta, 
      Edmonton, AB, T6G 2H7, Canada. sandra.davidge@ualberta.ca.
AD  - Women and Children's Health Research Institute, Edmonton, AB, T6G 2R3, Canada. 
      sandra.davidge@ualberta.ca.
LA  - eng
GR  - MOP132320/CIHR/Canada
GR  - FS154313/CIHR/Canada
GR  - MOP133675/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190702
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Blood Pressure
MH  - Cytokines/blood/metabolism
MH  - *Disease Susceptibility
MH  - Female
MH  - Inflammation Mediators
MH  - *Models, Biological
MH  - Oxidative Stress
MH  - Placenta/*blood supply/*metabolism
MH  - Pre-Eclampsia/diagnosis/*etiology/*metabolism/physiopathology
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - Proteinuria
MH  - Rats
MH  - Regional Blood Flow
MH  - Translational Research, Biomedical
MH  - Uterus/*blood supply/*metabolism
PMC - PMC6606748
COIS- The authors declare no competing interests.
EDAT- 2019/07/04 06:00
MHDA- 2020/10/23 06:00
PMCR- 2019/07/02
CRDT- 2019/07/04 06:00
PHST- 2019/02/12 00:00 [received]
PHST- 2019/06/20 00:00 [accepted]
PHST- 2019/07/04 06:00 [entrez]
PHST- 2019/07/04 06:00 [pubmed]
PHST- 2020/10/23 06:00 [medline]
PHST- 2019/07/02 00:00 [pmc-release]
AID - 10.1038/s41598-019-45959-6 [pii]
AID - 45959 [pii]
AID - 10.1038/s41598-019-45959-6 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jul 2;9(1):9565. doi: 10.1038/s41598-019-45959-6.