PMID- 31267427
OWN - NLM
STAT- MEDLINE
DCOM- 20200122
LR  - 20200122
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1984
DP  - 2019
TI  - Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH.
PG  - 117-122
LID - 10.1007/978-1-4939-9432-8_14 [doi]
AB  - Ionizing radiation can produce many chromosome aberrations; however, a number of 
      aberrations are not readily detectable with a conventional Giemsa staining and 
      require special staining methods. Moreover, an easy method to identify 
      nondetectable chromosomal aberrations, such as symmetrical inter-chromosomal 
      translocations, is available: whole chromosome fluorescence in situ hybridization 
      (FISH) staining. Asymmetrical translocations such as dicentric chromosomes are 
      known to be lethal but cells with symmetrical translocations may survive and 
      persist in the progeny population for years. Chromosome banding techniques can 
      also detect inter-chromosomal translocations but understanding banding patterns 
      requires professional training and experience. Due to its clear detection by a 
      discernible painting color, whole chromosome painting method is superior in 
      identifying chromosome fragmentation, loss, and amplification of chromosomes. 
      This chapter introduces whole chromosome FISH painting method to detect 
      chromosomal aberrations such asfragments and translocations.
FAU - Haskins, Jeremy S
AU  - Haskins JS
AD  - Department of Environmental and Radiological Health Sciences, Colorado State 
      University, Fort Collins, CO, USA.
FAU - Kato, Takamitsu A
AU  - Kato TA
AD  - Department of Environmental and Radiological Health Sciences, Colorado State 
      University, Fort Collins, CO, USA. Takamitsu.kato@colostate.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Painting/*methods
MH  - DNA Probes/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - Denature
OT  - FISH
OT  - Fragments
OT  - Reciprocal translocation
OT  - Whole chromosome painting
EDAT- 2019/07/04 06:00
MHDA- 2020/01/23 06:00
CRDT- 2019/07/04 06:00
PHST- 2019/07/04 06:00 [entrez]
PHST- 2019/07/04 06:00 [pubmed]
PHST- 2020/01/23 06:00 [medline]
AID - 10.1007/978-1-4939-9432-8_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1984:117-122. doi: 10.1007/978-1-4939-9432-8_14.