PMID- 31270009
OWN - NLM
STAT- MEDLINE
DCOM- 20200810
LR  - 20200810
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 31
IP  - 11
DP  - 2019 Oct
TI  - Expression and localisation of epidermal growth factor receptors and their 
      ligands in the lower genital tract of cycling cows.
PG  - 1692-1706
LID - 10.1071/RD18179 [doi]
AB  - The epidermal growth factor receptor (ErbB) family and its ligands are essential 
      for the regulation of multiple cellular processes required for mammalian 
      reproduction. The objectives of this study were to investigate the expression and 
      localisation of ErbB subtypes (ErbB1-4) and selected ligands, namely epidermal 
      growth factor (EGF), amphiregulin (AREG) and neuregulin (NRG), in the cervix and 
      vagina of cycling cows and to determine possible steroid hormone-dependence of 
      their expression using immunohistochemistry. All four ErbBs and EGF, AREG and NRG 
      proteins were found to be localised in the nucleus and cytoplasm of different 
      cells in the cervix and vagina, and their expression differed during the oestrous 
      cycle. During the follicular phase, in both the cervix and vagina, ErbB1, ErbB2, 
      ErbB3, ErbB4 and EGF expression was higher in the luminal epithelium (LE) than in 
      stromal and smooth muscle (SM) cells (P<0.05). During the luteal phase, the 
      expression of ErbB1, ErbB3 and EGF in the LE was significantly different from 
      that in stromal and SM cells in the cervix, whereas the expression of EGF and 
      AREG differed in the vagina compared to the cervix (P<0.05). Throughout the 
      oestrous cycle, in both the cervix and vagina, although ErbB2/human epidermal 
      growth factor receptor 2 expression in the LE and SM cells was significantly 
      higher than in the stromal cells (P<0.05), NRG expression was similar in the LE, 
      stromal and SM cells (P>0.05). Overall, these results suggest that all four ErbBs 
      and the EGF, AREG and NRG proteins may collectively contribute to several 
      cellular processes in the bovine cervix and vagina during the oestrous cycle.
FAU - Sa Soz, Hakan
AU  - Sa Soz H
AD  - Dicle University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 21280, Diyarbakir, Turkey; and Corresponding author. Email: 
      hakan.sagsoz@dicle.edu.tr.
FAU - Liman, Narin
AU  - Liman N
AD  - Erciyes University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 38039, Kayseri, Turkey.
FAU - Guney Saruhan, Berna
AU  - Guney Saruhan B
AD  - Dicle University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 21280, Diyarbakir, Turkey.
FAU - Akbal K, Mehmet E
AU  - Akbal K ME
AD  - Dicle University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 21280, Diyarbakir, Turkey.
FAU - Ketani, Muzaffer A
AU  - Ketani MA
AD  - Dicle University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 21280, Diyarbakir, Turkey.
FAU - Topalo Lu, U Ur
AU  - Topalo Lu UU
AD  - Dicle University, Faculty of Veterinary Medicine, Department of Histology and 
      Embryology, 21280, Diyarbakir, Turkey.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (EGF Family of Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - EGF Family of Proteins/metabolism
MH  - Epidermal Growth Factor/*metabolism
MH  - Epithelial Cells/metabolism
MH  - Epithelium/metabolism
MH  - ErbB Receptors/*metabolism
MH  - Estrous Cycle/*physiology
MH  - Female
MH  - Genitalia, Female/*metabolism
MH  - Immunohistochemistry
MH  - Uterus/metabolism
MH  - Vagina/metabolism
EDAT- 2019/07/05 06:00
MHDA- 2020/08/11 06:00
CRDT- 2019/07/05 06:00
PHST- 2018/05/11 00:00 [received]
PHST- 2019/06/10 00:00 [accepted]
PHST- 2019/07/05 06:00 [pubmed]
PHST- 2020/08/11 06:00 [medline]
PHST- 2019/07/05 06:00 [entrez]
AID - RD18179 [pii]
AID - 10.1071/RD18179 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2019 Oct;31(11):1692-1706. doi: 10.1071/RD18179.