PMID- 31271131
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20190704
IS  - 1550-7033 (Print)
IS  - 1550-7033 (Linking)
VI  - 13
IP  - 11
DP  - 2017 Nov 1
TI  - Local Delivery of BMP-2 from Poly(lactic-co-glycolic acid) Microspheres 
      Incorporated into Porous Nanofibrous Scaffold for Bone Tissue Regeneration.
PG  - 1446-1456
LID - 10.1166/jbn.2017.2445 [doi]
AB  - The integration of porous scaffold and growth factor is widely accepted as an 
      emerging and powerful strategy for bone tissue regeneration. Herein, we 
      fabricated the polymeric scaffold with macroporous and nanofibrous architecture 
      that composed of poly(L-lactic acid) (PLLA), poly(lactic-co-glycolic acid) (PLGA) 
      and polycaprolactone (PCL) using thermally induced phase separation (TIPS) 
      technique. The bone morphogenetic protein-2 (BMP-2) loaded PLGA microspheres (MS) 
      were prepared and then incorporated into nanofibrous scaffold (NF scaffold) to 
      obtain the BMP-2-loaded composite scaffold (BMP-2@MS/NF scaffold). The in vitro 
      release behavior of BMP-2 from the composite scaffold showed a sustained release 
      of over 5 weeks. The mouse primary osteoblasts (mOBs) grown on these BMP-2-loaded 
      composite scaffolds showed better adhesion and proliferation than those grown on 
      the scaffolds without BMP-2 loading. Also, the BMP-2-loaded composite scaffolds 
      displayed enhanced in vitro osteogenic differentiation of osteoblasts, as 
      evidenced by the results of alkaline phosphatase (ALP) activity and Alizarin Red 
      S staining. In addition, histology from an in vivo osteogenic potential 
      evaluation from a rat calvarial defect model demonstrated high osteoinductivity 
      of these BMP-2-loaded composite scaffolds. These results suggested that 
      constructed BMP-2-loaded composite scaffold had the potential to be used as a 
      bone implant, whose osteogenic ability was promoted by the prolonged release of 
      BMP-2.
FAU - Wang, Weizhong
AU  - Wang W
FAU - Miao, Yingke
AU  - Miao Y
FAU - Zhou, Xiaojun
AU  - Zhou X
FAU - Nie, Wei
AU  - Nie W
FAU - Chen, Liang
AU  - Chen L
FAU - Liu, Dinghua
AU  - Liu D
FAU - Du, Haibo
AU  - Du H
FAU - He, Chuanglong
AU  - He C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Nanotechnol
JT  - Journal of biomedical nanotechnology
JID - 101230869
EDAT- 2017/11/01 00:00
MHDA- 2017/11/01 00:01
CRDT- 2019/07/05 06:00
PHST- 2019/07/05 06:00 [entrez]
PHST- 2017/11/01 00:00 [pubmed]
PHST- 2017/11/01 00:01 [medline]
AID - 10.1166/jbn.2017.2445 [doi]
PST - ppublish
SO  - J Biomed Nanotechnol. 2017 Nov 1;13(11):1446-1456. doi: 10.1166/jbn.2017.2445.