PMID- 31271797
OWN - NLM
STAT- MEDLINE
DCOM- 20200115
LR  - 20200115
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 137
DP  - 2019 Sep 15
TI  - Enhanced cutaneous wound healing by functional injectable thermo-sensitive 
      chitosan-based hydrogel encapsulated human umbilical cord-mesenchymal stem cells.
PG  - 433-441
LID - S0141-8130(19)31564-8 [pii]
LID - 10.1016/j.ijbiomac.2019.06.246 [doi]
AB  - Human umbilical cord-mesenchymal stem cells (hUCMSCs) can secrete a variety of 
      cytokines and growth factors promoting wound repair. Hydrogel is suitable 
      biomaterial to supply niche for cells adhesion and survival. This study 
      constructed a functional injectable thermo-sensitive hydrogel (chitosan/glycerol 
      phosphate sodium/cellulose nanocrystals, CS/GP/CNC) encapsulated hUCMSCs to 
      repair full-thickness cutaneous wound. Addition of CNC to the CS/GP system not 
      only accelerated the gel speed, but also greatly improved the mechanical 
      properties of the gel and decreased degradation rate. The novel hydrogel was 
      injectable and low toxicity. Histological detection showed that hydrogel-hUCMSCs 
      combination significantly accelerated wound closure, microcirculation, tissue 
      remodeling, re-epithelialization and hair follicle regeneration, and inhibited 
      over-inflammation in the central and surrounding wounds. The hydrogel-hUCMSCs 
      combination promoted collagen deposition and keratinocyte mature marker K1 
      expression, decreased inflammatory factors secretion namely TNF-alpha and IL-1beta. The 
      present data provides a potential strategy for treatment of non-healing chronic 
      cutaneous wounds.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Xu, Hongjie
AU  - Xu H
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, 
      Guangdong Provincial Engineering and Technological Research Center for Drug 
      Carrier Development, Department of Biomedical Engineering, Jinan University, 
      Guangzhou 510632, China; Beogene Biotech (Guangzhou) Co., Ltd, Guangzhou 510663, 
      China.
FAU - Huang, Shanghui
AU  - Huang S
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, 
      Guangdong Provincial Engineering and Technological Research Center for Drug 
      Carrier Development, Department of Biomedical Engineering, Jinan University, 
      Guangzhou 510632, China.
FAU - Wang, Jingjing
AU  - Wang J
AD  - Department of Neurology, Weihai Central Hospital, Weihai 264400, China.
FAU - Lan, Yong
AU  - Lan Y
AD  - Beogene Biotech (Guangzhou) Co., Ltd, Guangzhou 510663, China.
FAU - Feng, Longbao
AU  - Feng L
AD  - Beogene Biotech (Guangzhou) Co., Ltd, Guangzhou 510663, China.
FAU - Zhu, Meishu
AU  - Zhu M
AD  - Department of Burn & Plastic Surgery, the First Affiliated Hospital of Shenzhen 
      University, the Second People's Hospital of Shenzhen, Shenzhen 518035, China.
FAU - Xiao, Yang
AU  - Xiao Y
AD  - Stem Cell Translational Medicine Center, the Second Affiliated Hospital of 
      Guangzhou Medical University, Guangzhou 510260, China.
FAU - Cheng, Biao
AU  - Cheng B
AD  - Center of Wound Treatment, General Hospital of Southern Theater Command, PLA, 
      Guangzhou 510010, China.
FAU - Xue, Wei
AU  - Xue W
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, 
      Guangdong Provincial Engineering and Technological Research Center for Drug 
      Carrier Development, Department of Biomedical Engineering, Jinan University, 
      Guangzhou 510632, China. Electronic address: weixue_jnu@aliyun.com.
FAU - Guo, Rui
AU  - Guo R
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, 
      Guangdong Provincial Engineering and Technological Research Center for Drug 
      Carrier Development, Department of Biomedical Engineering, Jinan University, 
      Guangzhou 510632, China. Electronic address: guorui@jnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190702
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Capsules)
RN  - 0 (Hydrogels)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Capsules
MH  - Cell Differentiation/drug effects
MH  - Chitosan/*chemistry
MH  - Humans
MH  - Hydrogels/*chemistry/pharmacology
MH  - Injections
MH  - Mesenchymal Stem Cells/*cytology
MH  - Neovascularization, Physiologic/drug effects
MH  - Rheology
MH  - *Skin/drug effects
MH  - *Temperature
MH  - Umbilical Cord/*cytology/drug effects
MH  - *Wound Healing
OTO - NOTNLM
OT  - Cellulose nanocrystals
OT  - Chitosan
OT  - Cutaneous wound
OT  - Injectable thermo-sensitive hydrogel
OT  - Mesenchymal stem cells
EDAT- 2019/07/05 06:00
MHDA- 2020/01/16 06:00
CRDT- 2019/07/05 06:00
PHST- 2019/03/01 00:00 [received]
PHST- 2019/04/08 00:00 [revised]
PHST- 2019/06/30 00:00 [accepted]
PHST- 2019/07/05 06:00 [pubmed]
PHST- 2020/01/16 06:00 [medline]
PHST- 2019/07/05 06:00 [entrez]
AID - S0141-8130(19)31564-8 [pii]
AID - 10.1016/j.ijbiomac.2019.06.246 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Sep 15;137:433-441. doi: 
      10.1016/j.ijbiomac.2019.06.246. Epub 2019 Jul 2.