PMID- 31272097
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20231113
IS  - 1423-0151 (Electronic)
IS  - 1011-7571 (Print)
IS  - 1011-7571 (Linking)
VI  - 29
IP  - 1
DP  - 2020
TI  - Association of HLA Alleles with Primary Sjogren Syndrome in the South Tunisian 
      Population.
PG  - 32-38
LID - 10.1159/000501896 [doi]
AB  - OBJECTIVE: In order to investigate human leukocyte antigen (HLA) genes 
      predisposing to primary Sjogren syndrome (pSS), we conducted an association study 
      using HLA loci (A, B, and DRB1) and 9 polymorphic microsatellite markers spanning 
      the HLA region in pSS patients as compared to healthy individuals. SUBJECTS AND 
      METHODS: Forty-four patients fitting the European criteria of pSS and 123 healthy 
      controls were analyzed for their HLA class I and class II alleles. HLA class I 
      typing was performed using a standard microlymphocytotoxicity method followed by 
      PCR-SSP. HLA-DRB1 genotyping was performed using PCR-SSP. We studied the 
      polymorphism of 9 microsatellite markers for both groups. Microsatellite 
      genotyping was performed using the PCR fluorescent technique. RESULTS: We 
      observed a positive association between HLA-B15 and pSS in the Tunisian 
      population (p = 0.004, OR 7.57). The comparison of the frequencies of DRB1 
      alleles in pSS patients and controls confirmed the association of the DRB1*03 
      allele with pSS (p = 0.02, OR 2.36). On the other hand, the association study of 
      microsatellite markers showed that the a9 allele of D6S265 marker and the a20 of 
      C1.2.C were found to be positively associated with pSS as compared to controls (p 
      =0.0003, OR 10.29, and p =0.001, OR 4.79, respectively). Using the "Haplo.stats" 
      software analysis, we found that the most associated region was located in the 
      HLA class I region and limited by HLA-A and D6S265 loci (p = 0.00056). 
      CONCLUSION: The results of this study support the hypothesis of the existence of 
      a susceptibility gene for pSS located in the HLA class I and III regions.
CI  - (c) 2019 The Author(s) Published by S. Karger AG, Basel.
FAU - Charfi, Aida
AU  - Charfi A
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia, 
      aydack@gmail.com.
FAU - Mahfoudh, Nadia
AU  - Mahfoudh N
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Kamoun, Arwa
AU  - Kamoun A
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Frikha, Feten
AU  - Frikha F
AD  - Department of Internal Medicine, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Dammak, Chifa
AU  - Dammak C
AD  - Department of Internal Medicine, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Gaddour, Lilia
AU  - Gaddour L
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Hakim, Feiza
AU  - Hakim F
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Maalej, Leila
AU  - Maalej L
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Mallek, Bakhta
AU  - Mallek B
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Kammoun, Ines
AU  - Kammoun I
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Bahloul, Zouhir
AU  - Bahloul Z
AD  - Department of Internal Medicine, Hedi Chaker University Hospital, Sfax, Tunisia.
FAU - Makni, Hafedh
AU  - Makni H
AD  - Department of Histocompatibility, Hedi Chaker University Hospital, Sfax, Tunisia.
LA  - eng
PT  - Journal Article
DEP - 20190705
PL  - Switzerland
TA  - Med Princ Pract
JT  - Medical principles and practice : international journal of the Kuwait University, 
      Health Science Centre
JID - 8901334
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-B Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Case-Control Studies
MH  - Female
MH  - Genotype
MH  - HLA Antigens/*genetics
MH  - HLA-B Antigens/genetics
MH  - Humans
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Sjogren's Syndrome/*genetics
MH  - Tunisia
MH  - Young Adult
PMC - PMC7024883
OTO - NOTNLM
OT  - Haplotype
OT  - Human leukocyte antigen
OT  - Microsatellite
OT  - Polymorphism
OT  - Primary Sjogren syndrome
COIS- The authors have no conflicts of interest to declare.
EDAT- 2019/07/05 06:00
MHDA- 2021/01/23 06:00
PMCR- 2019/07/05
CRDT- 2019/07/05 06:00
PHST- 2018/07/25 00:00 [received]
PHST- 2019/07/04 00:00 [accepted]
PHST- 2019/07/05 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
PHST- 2019/07/05 06:00 [entrez]
PHST- 2019/07/05 00:00 [pmc-release]
AID - 000501896 [pii]
AID - mpp-0029-0032 [pii]
AID - 10.1159/000501896 [doi]
PST - ppublish
SO  - Med Princ Pract. 2020;29(1):32-38. doi: 10.1159/000501896. Epub 2019 Jul 5.