PMID- 31272281
OWN - NLM
STAT- MEDLINE
DCOM- 20200511
LR  - 20200704
IS  - 1555-8592 (Electronic)
IS  - 1547-6278 (Print)
IS  - 1547-6278 (Linking)
VI  - 15
IP  - 2
DP  - 2019
TI  - ATP6V1H facilitates osteogenic differentiation in MC3T3-E1 cells via Akt/GSK3beta 
      signaling pathway.
PG  - 43-54
LID - 10.1080/15476278.2019.1633869 [doi]
AB  - Type 2 diabetes mellitus (T2DM) accounts for approximately 90% of all diabetic 
      patients, and osteoporosis is one of the complications during T2DM process. 
      ATP6V1H (V-type proton ATPase subunit H) displays crucial roles in inhibiting 
      bone loss, but its role in osteogenic differentiation remains unknown. Therefore 
      in this study, we aimed to explore the biological role of ATP6V1H in osteogenic 
      differentiation. OM (osteogenic medium) and HG (high glucose and free fatty 
      acids) were used to induce the MC3T3-E1 cells into osteogenic differentiation in 
      a T2DM simulating environment. CCK8 assay was used to detect cell viability. 
      Alizarin Red staining was used to detect the influence of ATP6V1H on osteogenic 
      differentiation. ATP6V1H expression increased in OM-MC3T3-E1 cells, while 
      decreased in OM+HG-MC3T3-E1 cells. ATP6V1H promoted osteogenic differentiation of 
      OM+HG-MC3T3-E1 cells. Overexpression of ATP6V1H inhibited Akt/GSK3beta signaling 
      pathway, while knockdown of ATP6V1H promoted Akt/GSK3beta signaling pathway. ATP6V1H 
      overexpression promoted osteogenic differentiation of OM+HG-MC3T3-E1 cells. The 
      role of ATP6V1H in osteogenic differentiation in a T2DM simulating environment 
      involved in Akt/GSK3beta signaling pathway. These data demonstrated that ATP6V1H 
      could serve as a potential target for osteogenic differentiation in a T2DM 
      simulating environment.
FAU - Jiang, Fusong
AU  - Jiang F
AUID- ORCID: 0000-0002-2765-0041
AD  - a Department of Endocrinology and Metabolism, Shanghai Jiao Tong University 
      Affiliated Sixth People's Hospital, Shanghai Clinical Center for Diabetes , 
      Shanghai , China.
FAU - Shan, Haojie
AU  - Shan H
AD  - b Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Pan, Chenhao
AU  - Pan C
AD  - b Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Zhou, Zubin
AU  - Zhou Z
AD  - b Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
FAU - Cui, Keze
AU  - Cui K
AD  - c Department of Orthopaedic Surgery, Haikou Orthopedics and Diabetes Hospital of 
      Shanghai Sixth People's Hospital , Haikou , China.
FAU - Chen, Yuanliang
AU  - Chen Y
AD  - c Department of Orthopaedic Surgery, Haikou Orthopedics and Diabetes Hospital of 
      Shanghai Sixth People's Hospital , Haikou , China.
FAU - Zhong, Haibo
AU  - Zhong H
AD  - c Department of Orthopaedic Surgery, Haikou Orthopedics and Diabetes Hospital of 
      Shanghai Sixth People's Hospital , Haikou , China.
FAU - Lin, Zhibin
AU  - Lin Z
AD  - c Department of Orthopaedic Surgery, Haikou Orthopedics and Diabetes Hospital of 
      Shanghai Sixth People's Hospital , Haikou , China.
FAU - Wang, Nan
AU  - Wang N
AD  - d Department of Emergency, The First Affiliated Hospital of Zhengzhou University 
      , Zhengzhou , Henan , China.
FAU - Yan, Liang
AU  - Yan L
AD  - e Department of Ophthalmology, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital , Shanghai , China.
FAU - Yu, Xiaowei
AU  - Yu X
AD  - b Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated 
      Sixth People's Hospital , Shanghai , China.
LA  - eng
PT  - Journal Article
DEP - 20190704
PL  - United States
TA  - Organogenesis
JT  - Organogenesis
JID - 101253266
RN  - EC 2.7.11.1 (Akt1 protein, mouse)
RN  - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
RN  - EC 2.7.11.1 (Gsk3b protein, mouse)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.6.1.- (ATP6V1H protein, mouse)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Survival
MH  - *Gene Expression Regulation
MH  - Glycogen Synthase Kinase 3 beta/*metabolism
MH  - Mice
MH  - Osteoblasts/cytology
MH  - *Osteogenesis
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Signal Transduction
MH  - Vacuolar Proton-Translocating ATPases/*metabolism
PMC - PMC6668661
OTO - NOTNLM
OT  - ATP6V1H
OT  - Akt/GSK3beta
OT  - MC3T3-E1
OT  - Osteogenic Differentiation
OT  - T2DM
EDAT- 2019/07/06 06:00
MHDA- 2020/05/12 06:00
PMCR- 2020/07/04
CRDT- 2019/07/06 06:00
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2020/05/12 06:00 [medline]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2020/07/04 00:00 [pmc-release]
AID - 1633869 [pii]
AID - 10.1080/15476278.2019.1633869 [doi]
PST - ppublish
SO  - Organogenesis. 2019;15(2):43-54. doi: 10.1080/15476278.2019.1633869. Epub 2019 
      Jul 4.