PMID- 31273203
OWN - NLM
STAT- MEDLINE
DCOM- 20190910
LR  - 20210110
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 10
IP  - 1
DP  - 2019 Jul 4
TI  - Induction of memory-like dendritic cell responses in vivo.
PG  - 2955
LID - 10.1038/s41467-019-10486-5 [doi]
LID - 2955
AB  - Dendritic cells (DCs), a vital component of the innate immune system, are 
      considered to lack antigen specificity and be devoid of immunological memory. 
      Strategies that can induce memory-like responses from innate cells can be 
      utilized to elicit protective immunity in immune deficient persons. Here we 
      utilize an experimental immunization strategy to modulate DC inflammatory and 
      memory-like responses against an opportunistic fungal pathogen that causes 
      significant disease in immunocompromised individuals. Our results show that DCs 
      isolated from protectively immunized mice exhibit enhanced transcriptional 
      activation of interferon and immune signaling pathways. We also show long-term 
      memory-like cytokine responses upon subsequent challenge with the fungal pathogen 
      that are abrogated with inhibitors of specific histone modifications. Altogether, 
      our study demonstrates that immunization strategies can be designed to elicit 
      memory-like DC responses against infectious disease.
FAU - Hole, Camaron R
AU  - Hole CR
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Wager, Chrissy M Leopold
AU  - Wager CML
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Castro-Lopez, Natalia
AU  - Castro-Lopez N
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Campuzano, Althea
AU  - Campuzano A
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Cai, Hong
AU  - Cai H
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Wozniak, Karen L
AU  - Wozniak KL
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Wang, Yufeng
AU  - Wang Y
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA.
FAU - Wormley, Floyd L Jr
AU  - Wormley FL Jr
AD  - Department of Biology, The University of Texas at San Antonio, San Antonio, TX, 
      78249, USA. floyd.wormley@utsa.edu.
AD  - The South Texas Center for Emerging Infectious Diseases, The University of Texas 
      at San Antonio, San Antonio, TX, 78249, USA. floyd.wormley@utsa.edu.
LA  - eng
GR  - R01 AI071752/AI/NIAID NIH HHS/United States
GR  - R25 GM060655/GM/NIGMS NIH HHS/United States
GR  - 2RO1AI071752/U.S. Department of Health & Human Services | NIH | National 
      Institute of Allergy and Infectious Diseases (NIAID)/International
GR  - GM060655/U.S. Department of Health & Human Services | NIH | National 
      Institute of General Medical Sciences (NIGMS)/International
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190704
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Histones)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cryptococcosis/immunology/microbiology
MH  - Cryptococcus/physiology
MH  - Dendritic Cells/*immunology/microbiology
MH  - Female
MH  - Histones/metabolism
MH  - Immunity, Innate
MH  - *Immunologic Memory
MH  - Inflammation/genetics/pathology
MH  - Interferon-gamma/metabolism
MH  - Lung/immunology/microbiology
MH  - Mice, Inbred BALB C
MH  - Phenotype
MH  - Protein Processing, Post-Translational
MH  - RNA, Messenger/genetics/metabolism
MH  - Vaccination
PMC - PMC6609631
COIS- The authors declare no competing interests.
EDAT- 2019/07/06 06:00
MHDA- 2019/09/11 06:00
PMCR- 2019/07/04
CRDT- 2019/07/06 06:00
PHST- 2018/03/13 00:00 [received]
PHST- 2019/05/08 00:00 [accepted]
PHST- 2019/07/06 06:00 [entrez]
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2019/09/11 06:00 [medline]
PHST- 2019/07/04 00:00 [pmc-release]
AID - 10.1038/s41467-019-10486-5 [pii]
AID - 10486 [pii]
AID - 10.1038/s41467-019-10486-5 [doi]
PST - epublish
SO  - Nat Commun. 2019 Jul 4;10(1):2955. doi: 10.1038/s41467-019-10486-5.