PMID- 31274018
OWN - NLM
STAT- MEDLINE
DCOM- 20210615
LR  - 20211204
IS  - 1744-4160 (Electronic)
IS  - 1381-3455 (Linking)
VI  - 127
IP  - 1
DP  - 2021 Feb
TI  - Role of mammalian target of rapamycin in regulating HIF-1alpha and vascular 
      endothelial growth factor signals in glaucoma.
PG  - 44-50
LID - 10.1080/13813455.2019.1609996 [doi]
AB  - Hypoxia inducible factor subtype 1alpha (HIF-1alpha) in retinal tissues is involved in 
      the development of glaucoma. This study examined the role played by mammalian 
      target of rapamycin (mTOR) in regulating expression of HIF-1alpha and its downstream 
      pathway, vascular endothelial growth factor (VEGF). Glaucoma was induced by 
      chronic elevation of intraocular pressure using laser burns in rats. ELISA and 
      western blot analysis were employed to determine the levels of HIF-1alpha, VEGF and 
      mTOR in retinal tissues of eyes with high intraocular pressure. In results, 
      HIF-1alpha, VEGF and VEGF receptor subtype 2 were increased in laser eyes. The 
      p-mTOR, mTOR-mediated phosphorylation of 4E-binding protein 4, p70 ribosomal S6 
      protein kinase 1 were also amplified in retina of laser eyes. Blocking mTOR using 
      rapamycin attenuated HIF-1alpha-VEGF pathways, accompanied with downregulation of 
      apoptotic Caspase-3. Our data revealed potential signalling pathways engaged in 
      the development of glaucoma, including the activation of mTOR and HIF-1alpha-VEGF 
      mechanism.
FAU - Zhou, Jinzi
AU  - Zhou J
AD  - Department of Ophthalmology, The First People's Hospital of Guiyang, Guiyang, 
      China.
FAU - Chen, Fenghua
AU  - Chen F
AD  - Department of Ophthalmology, The First People's Hospital of Guiyang, Guiyang, 
      China.
FAU - Yan, Aimin
AU  - Yan A
AD  - Department of Ophthalmology, The First People's Hospital of Guiyang, Guiyang, 
      China.
FAU - Xia, Xiaobo
AU  - Xia X
AD  - Department of Ophthalmology, Xiangya Hospital of Central South University, 
      Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20190705
PL  - England
TA  - Arch Physiol Biochem
JT  - Archives of physiology and biochemistry
JID - 9510153
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Caspase 3/metabolism
MH  - *Gene Expression Regulation
MH  - Glaucoma/*metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Intraocular Pressure
MH  - Male
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Vascular Endothelial Growth Factor A/*metabolism
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
OTO - NOTNLM
OT  - HIF-1alpha
OT  - VEGF
OT  - glaucoma
OT  - mTOR
EDAT- 2019/07/06 06:00
MHDA- 2021/06/16 06:00
CRDT- 2019/07/06 06:00
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2021/06/16 06:00 [medline]
PHST- 2019/07/06 06:00 [entrez]
AID - 10.1080/13813455.2019.1609996 [doi]
PST - ppublish
SO  - Arch Physiol Biochem. 2021 Feb;127(1):44-50. doi: 10.1080/13813455.2019.1609996. 
      Epub 2019 Jul 5.