PMID- 31274405
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20190909
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Linking)
VI  - 69
IP  - 9
DP  - 2019 Sep
TI  - Acidithiobacillus sulfuriphilus sp. nov.: an extremely acidophilic 
      sulfur-oxidizing chemolithotroph isolated from a neutral pH environment.
PG  - 2907-2913
LID - 10.1099/ijsem.0.003576 [doi]
AB  - The genus Acidithiobacillus currently includes seven species with validly 
      published names, which fall into two major groups, those that can oxidize ferrous 
      iron and those that do not. All seven species can use zero-valent sulfur and 
      reduced sulfur oxy-anions as electron donors, are obligately chemolithotrophic 
      and acidophilic bacteria with pH growth optima below 3.0. The 16S rRNA gene of a 
      novel strain (CJ-2(T)) isolated from circum-neutral pH mine drainage showed 
      95-97 % relatedness to members of the genus Acidithiobacillus. Digital DNA-DNA 
      hybridization (dDDH) values between strains and whole-genome pairwise comparisons 
      between the CJ-2(T) strain and the reference genomes available for members of the 
      genus Acidithiobacillus confirmed that CJ-2(T)represents a novel species of this 
      genus. CJ-2(T) is a strict aerobe, oxidizes zero-valent sulfur and reduced 
      inorganic sulfur compounds but does not use ferrous iron or hydrogen as electron 
      donors. The isolate is mesophilic (optimum growth temperature 25-28  degrees C) and 
      extremely acidophilic (optimum growth pH 3.0), though its pH optimum and maximum 
      were significantly higher than those of non-iron-oxidising acidithiobacilli with 
      validly published names. The major fatty acids of CJ-2(T) were C18 : 1omega7c, 
      C:16 : 1omega7c/iso-C15 : 0 2-OH, C16 : 0 and C19 : 0 cyclo omega8c and the major 
      respiratory quinone present was Q8. The name Acidithiobacillussulfuriphilus sp. 
      nov. is proposed, the type strain is CJ-2(T) (=DSM 105150(T)=KCTC 4683(T)).
FAU - Falagan, Carmen
AU  - Falagan C
AD  - Present address: Environmental Sustainability Institute and Camborne School of 
      Mines, College of Engineering, Mathematics and Physical Sciences, University of 
      Exeter, UK.
AD  - School of Biological Sciences, Bangor University, Bangor LL57 2UW, UK.
FAU - Moya-Beltran, Ana
AU  - Moya-Beltran A
AD  - Fundacion Ciencia y Vida, Avenida Zanartu 1482, Nunoa, Santiago, Chile.
AD  - Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile.
AD  - Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.
FAU - Castro, Matias
AU  - Castro M
AD  - Fundacion Ciencia y Vida, Avenida Zanartu 1482, Nunoa, Santiago, Chile.
AD  - Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile.
FAU - Quatrini, Raquel
AU  - Quatrini R
AD  - Millennium Nucleus in the Biology of Intestinal Microbiota, Santiago, Chile.
AD  - Fundacion Ciencia y Vida, Avenida Zanartu 1482, Nunoa, Santiago, Chile.
FAU - Johnson, D Barrie
AU  - Johnson DB
AD  - School of Biological Sciences, Bangor University, Bangor LL57 2UW, UK.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 70FD1KFU70 (Sulfur)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Acidithiobacillus/*classification/isolation & purification
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - DNA, Bacterial/genetics
MH  - Fatty Acids/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Iron
MH  - *Mining
MH  - Nucleic Acid Hybridization
MH  - Oxidation-Reduction
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sulfur/*metabolism
MH  - Wales
MH  - *Water Microbiology
OTO - NOTNLM
OT  - Acidithiobacillus
OT  - Acidithiobacillus sulfuriphilus
OT  - acidophile
OT  - pH neutral
OT  - sulfur-oxidation
EDAT- 2019/07/06 06:00
MHDA- 2019/09/10 06:00
CRDT- 2019/07/06 06:00
PHST- 2019/07/06 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2019/07/06 06:00 [entrez]
AID - 10.1099/ijsem.0.003576 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2019 Sep;69(9):2907-2913. doi: 10.1099/ijsem.0.003576.